Table 2 Gene specific forward and reverse primer sequences used for RT-PCR
From: Suppressive actions of eicosapentaenoic acid on lipid droplet formation in 3T3-L1 adipocytes
Gene | Forward primer (5' - 3') | Reverse primer (5' - 3') | NCBI accession number |
|---|---|---|---|
Adiponectin | AAGGACAAGGCCGTTCTCT | TATGGGTAGTTGCAGTCAGTTGG | NM_009605 |
Cidea | TGCTCTTCTGTATCGCCCAGT | GCCGTGTTAAGGAATCTGCTG | NM_007702 |
Cyclophilin | TCTGCTGTCTTTGGAACTTTGTC | CTGATGGCGAGCCCTTG | NM_008907 |
D9D/SCD1 | ATGCCGGCCCACATGCTCCAA | TCAGCTACTCTTGTGACTCCC | NM_009127 |
GLUT4 | TGTGGCCTTCTTTGAGATTGG | CTGAAGAGCTCGGCCCACAA | NM_009204 |
HSL | CCTACTGCTGGGCTGTCAA | CCATCTCGCACCCTCACT | NM_010719 |
IL-6 | ACAAGTCGGAGGCTTAATTAGACAT | TTGCCATTGCACAACTCTATTC | NM_031168 |
Leptin | TCCAGAAAGTCCAGGATGACAC | CACATTTTGGGAAGGCAGG | NM_008493 |
LPL | AGTAGACTGGTTGTATCGGG | AGCGTCATCAGGAGAAAGG | NM_008509 |
MCP-1 | CTTCCTCCACCACCATGCA | CCAGCCGGCAACTGTGA | NM_011333 |
Perilipin A | TGCTGGATGGAGACCTC | ACCGGCTCCATGCTCCA | NM_001113471 |
PPARγ | GGAATGGGAGTGGTCATCCA | CCCACCAACTTCGGAATC | NM_001127330 |