Figure 3From: When cholesterol is not cholesterol: a note on the enzymatic determination of its concentration in model systems containing vegetable extractsVegetable extracts interfere with cholesterol analyses in in the presence of porcine bile based systems using cholesterol oxidase peroxidase-coupled reactions. Both cocoa (a) and tea-derived (b) extracts inhibited, in a dose dependent fashion, bile-derived cholesterol reactivity in systems used for cholesterol analyses using cholesterol oxidase-peroxidase coupled reactions. Cholesterol-dependent and independent fluorescences were as defined in figure 2. Linearity of a cholesterol standard curve was sensible to the presence of either cocoa (c) and tea-derived (d) extracts diluted in bile in different concentrations (from 0 to 20 mg/ml). (e) Vegetable-extracts (10 mg/mL) inhibited cholesterol oxidase activity in the presence of bile, based on the cholesterol chromatographic assay. Values are means ± SEM. Statistical analysis was done by ANOVA followed by Tukey HSD post hoc test (* p < 0.05).Back to article page