Figure 4From: Fish oil supplementation reverses the effect of cholesterol on apoptotic gene expression in smooth muscle cells25-hidroxycholesterol effects on caspase-3 expresión in the cell culture model. caspase-3 mRNA quantification in SMC cultures (SMC-C, SMC-Ch, SMC-Ch-FO) and SMC cultures treated with 20 μg/mL 25-hydroxycholesterol for 24 h. mRNA levels were quantified by semiquantitative real-time reverse-transcription PCR. Results are shown as relative expression ratio of caspase-3 in SMC cultures with respect to control culture and expressed in comparison to reference gene β-actin. *P < 0.05, ** P < 0.01, *** P < 0.001 vs. SMC-C, +P < 0.05, ++P < 0.01, +++ P < 0.001 vs. SMC-C treated with 20 μg/mL 25-hydroxycholesterol.Back to article page