Figure 1

ApoA-I stimulates total cholesterol release. Panel A: Total cholesterol release was measured in the medium of mature adipocytes treated or not with apoA-I (5, 10 or 20 μg/ml) at 4, 6, 12 and 24 hours by fluorometric dosage. The graph represents the mean ± SD of the results from 4 patients (n = 6 for each condition, for each patient) and all raw data has been normalised versus control. ***P < 0.001%, **P < 0.01%, *P < 0.05%, versus untreated cells. Panel B: ABCA1 gene expression was determined at 1.5, 3, 6 and 12 hours in mature adipocyte cultures, treated or not with apoA-I (10 μg/ml). The graph represents the mean ± SD of the results from one patient (n = 5 for each condition), representative of two experiments on two different patients.