Figure 2

ApoA-I induced cholesterol release is cAMP and PKA independent. Depending upon the conditions, mature adipocytes were pre-treated or not for 1 hour with ACi (20 μM), PKAi (40 nM) or rp-8-Br-cAMP (40 μM). Cell medium was removed and cells were either treated or not with ACi or PKAi, rp-8Br-cAMP, FSK (20 μM) and with or without apoA-I (10 μg/ml) for 30 min or 12 hours. Panel A: cAMP was quantified in adipocytes, treated or not with ACi and FSK at 30 min, by competitive ELISA. Panel B: total cholesterol was measured in the medium of mature adipocytes cultures, treated or not with ACi and FSK at 12 hours by fluorometric assay. Panel C: total cholesterol was measured in the medium of mature adipocytes cultures, treated with different inhibitors and with or without apoA-I at 12 hours by fluorometric assay. Graphs represent the mean ± SD of the results from 4 patients (n = 6 for each condition, for each patient) and all raw data has been normalised versus control. ***P < 0.001%, **P < 0.01% versus control cells and #P < 0.1% versus apoA-I treated cells.