Effects of rAAV2/1-CMV-GH1 and high-fat feeding on liver histology. Lipid infiltration or significant hepatocytes abnormalities were not observed in the CS or GS groups. Numerous vacuoles were present in hepatocytes in the CH group. In contrast, the GH group contained fewer and smaller vacuoles compared with the CH group. (haematoxylin/eosin staining; magnification ×40).