ω-3 Polyunsaturated fatty acids prevent pressure overload-induced ventricular dilation and decrease in mitochondrial enzymes despite no change in adiponectin

Table 3 Metabolic Parameters

	WILD TYPE				ADIPONECTIN-/-
	SHAM		TAC		SHAM		TAC
	STD	ω-3 PUFA	STD	ω-3 PUFA	STD	ω-3 PUFA	STD	ω-3 PUFA
Serum Adiponectin (μg·ml^-1)	7.94 ± 1.23	8.74 ± 1.35	7.72 ± 1.27	7.48 ± 1.23	1.70 ± 0.12	1.56 ± 0.10	1.50 ± 0.10	1.49 ± 0.10
Serum HMW Adiponectin (μg·ml^-1)	0.55 ± 0.03	0.78 ± 0.08#	0.63 ± 0.05	0.73 ± 0.08	0.18 ± 0.03	0.18 ± 0.03	0.19 ± 0.02	0.19 ± 0.03
Adiponectin mRNA in Epididymal Fat	1.00 ± 0.17	1.10 ± 0.10	1.04 ± 0.14	1.18 ± 0.27	0	0	0	0
PDK4 mRNA in LV	1.00 ± 0.35	0.30 ± 0.03#	0.29 ± 0.04*	0.29 ± 0.04	0.27 ± 0.03	0.40 ± 0.11	0.37 ± 0.08	0.48 ± 0.14
UCP3 mRNA in LV	1.00 ± 0.14	0.88 ± 0.13	0.39 ± 0.07*	0.47 ± 0.12	0.89 ± 0.12	0.96 ± 0.11	0.49 ± 0.09	0.59 ± 0.10
Serum Glucose (mM)	12.7 ± 0.6	11.6 ± 0.8	12.6 ± 1.0	11.4 ± 0.9	13.0 ± 0.9	12.7 ± 0.7	11.7 ± 0.4	13.1 ± 1.1
Serum FFA (mM)	0.69 ± 0.05	0.55 ± 0.04	0.76 ± 0.14	0.55 ± 0.07	0.82 ± 0.08	0.47 ± 0.06	0.61 ± 0.07	0.52 ± 0.08
Serum Insulin (ng·ml^-1)	0.66 ± 0.16	0.90 ± 0.20	0.66 ± 0.16	0.48 ± 0.10	0.84 ± 0.23	0.54 ± 0.11	0.60 ± 0.10	0.34 ± 0.10
Serum Leptin (ng·ml^-1)	2.56 ± 0.51	2.80 ± 0.70	1.62 ± 0.37	1.18 ± 0.28*	3.83 ± 1.50	2.28 ± 0.82	1.85 ± 0.43	0.78 ± 0.14
Serum Triglycerides (mg·ml^-1)	0.26 ± 0.05	0.25 ± 0.06	0.25 ± 0.07	0.28 ± 0.06	0.40 ± 0.09	0.23 ± 0.08	0.30 ± 0.04	0.25 ± 0.06

Values are mean ± SEM; n = 12-15/group. *P < 0.05 vs. sham, #P < 0.05 vs. standard chow diet. HMW, high molecular weight; PDK4, pyruvate dehydrogenase kinase 4; UCP3, uncoupling protein 3; FFA, free fatty acids; LV, left ventricle; TAC, transverse aortic constriction; STD, standard chow. mRNA data are expressed as fold change relative to the mean of the wild type sham standard chow group.

ISSN: 1476-511X