Omega-6 effects of transfection with Δ4-desaturase on grouped controls and patients with the Zellweger syndrome. This figure displays the number of times that the 22:5ω6/22:4ω6 ratio increased when adding the Δ4-desaturase, in normal children, as well as in the ZS patients. A new index was obtained by dividing the 22:5ω6/22:4ω6 ratio with the Δ4-desaturase added by the same ratio without the enzyme [(22:5ω6/22:4ω6)+Δ4/(22:5ω6/22:4ω6) empty]. Bars are mean values ± SEM. Despite the effect of grouping patients and controls by unpaired t-tests, the statistical significance was still very high (p = 0.0009).