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Table 3 Fatty acid composition of lymphoblastoid cell lines transfected with the Δ4-desaturase vector, in healthy controls and in patients with the Zellweger syndrome.

From: The Δ4-desaturation pathway for DHA biosynthesis is operative in the human species: Differences between normal controls and children with the Zellweger syndrome

 

Controls

Zellweger syndrome

 

Fatty acid

Mean ± SEM

N

Mean ± SEM

N

Statistics

12:0

0.12 ± 0.021

16

0.24 ± 0.057

14

n.s.

14:0

1.75 ± 0.124

16

1.80 ± 0.103

14

n.s.

16:0

21.49 ± 0.239

16

21.58 ± 0.396

14

n.s.

16:1ω9

2.13 ± 0.096

16

1.99 ± 0.066

14

n.s.

16:1ω7

1.12 ± 0.077

16

1.12 ± 0.066

14

n.s.

18:0

15.60 ± 0.202

16

16.83 ± 0.326

14

P < 0.005

18:1ω9

18.45 ± 0.439

16

17.25 ± 0.756

14

n.s.

18:1ω7

7.09 ± 0.136

16

6.34 ± 0.265

14

n.s.

18:2ω6

2.73 ± 0.122

16

2.43 ± 0.085

14

n.s.

18:3ω6

0.11 ± 0.020

16

0.05 ± 0.015

14

n.s.

18:3ω3

0.06 ± 0.012

16

0.04 ± 0.009

14

n.s.

20:0

0.31 ± 0.050

16

0.27 ± 0.016

14

n.s.

20:1ω9

1.37 ± 0.071

16

1.69 ± 0.179

14

n.s.

20:2ω6

0.37 ± 0.019

16

0.39 ± 0.017

14

n.s.

20:3ω9

0.70 ± 0.065

16

0.64 ± 0.050

14

n.s.

20:3ω6

3.39 ± 0.083

16

2.68 ± 0.145

14

P = 0.0001

20:4ω6

7.69 ± 0.306

16

8.34 ± 0.681

14

n.s.

20:5ω3

0.56 ± 0.049

16

0.55 ± 0.073

14

n.s.

22:0

0.32 ± 0.023

16

0.30 ± 0.022

14

n.s.

22:1ω9

0.25 ± 0.017

16

0.39 ± 0.021

14

P < 0.0001

22:4ω6

1.19 ± 0.078

16

1.57 ± 0.139

14

P < 0.05

22:5ω6

0.56 ± 0.031

16

0.75 ± .053

14

P < 0.005

22:5ω3

3.58 ± 0.202

16

3.21 ± 0.157

14

n.s.

22:6ω3

5.56 ± 0.149

16

5.63 ± 0.159

14

n.s.

24:0

0.37 ± 0.011

16

0.47 ± 0.053

14

n.s.

24:1ω9

0.79 ± 0.072

16

0.86 ± 0.057

14

n.s.

26:0

0.06 ± 0.018

16

0.16 ± 0.029

14

P < 0.01

26:1ω9

0.11 ± 0.012

16

0.33 ± 0.027

14

P < 0.0001

24:4ω6

0.02 ± 0.008

16

0.01 ± 0.007

13

n.s.

24:5ω6

0.02 ± 0.011

16

0.11 ± 0.019

13

P = 0,0002

24:5ω3

0.03 ± 0.007

16

0.01 ± 0.005

13

P < 0.05

24:6ω3

0.07 ± 0.015

16

0.12 ± 0.010

13

P < 0.05

22:6ω3/22:5ω3

1.62 ± 0.096

16

1.82 ± 0.129

14

n.s.

22:5ω6 22:4ω6

0.52 ± 0.048

16

0.53 ± 0.064

14

n.s.

  1. This table lists the mean values ± SEM of total fatty acids (mol%) and their main ratios in immortalized lymphocytes transfected with the Δ4-desturase plasmid (pIRES2EGFP-FlagΔ4), in normal controls as compared with Zellweger-syndrome patients. As in table 2, p values are only shown for statistically significant changes.