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Figure 4 | Lipids in Health and Disease

Figure 4

From: Modulation of endothelial cell integrity and inflammatory activation by commercial lipid emulsions

Figure 4

Effect of lipid emulsion supplementation on phosphorylation of NF-κB. Endothelial cells were pretreated with OO (A), SO (B), or FO (C) for 24 hours under standard tissue culture conditions, followed by a 4-hour stimulation with or without LPS (1 μg/ml). Cell lysates were electrophoretically separated in a 4-12% polyacrylamide gradient gel and transferred onto nitrocellulose membranes. A phospho-specific antibody to the p65 regulatory subunit of NF-κB assessed the relative activation state of the enzyme. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as the protein-loading control.

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