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Table 4 Fatty acid profile in FO-supplemented HAECs

From: Modulation of endothelial cell integrity and inflammatory activation by commercial lipid emulsions

Fatty acid

Vehicle

0.10%

1%

2%

5%

10%

8:0

0.18 ± 0.04

0.25 ± 0.03

0.52 ± 0.11*

0.20 ± 0.03

0.20 ± 0.03

0.08 ± 0.03*

10:0

0.24 ± 0.09

0.26 ± 0.06

0.46 ± 0.05*

0.15 ± 0.03

0.18 ± 0.08

0.11 ± 0.05

12:0

0.18 ± 0.02

0.13 ± 0.00*

0.31 ± 0.06*

0.14 ± 0.01

0.14 ± 0.09

0.06 ± 0.02*

14:0

3.61 ± 0.18

4.27 ± 0.79

3.96 ± 1.06

5.35 ± 0.20*

1.47 ± 0.10*

1.05 ± 0.06*

16:0

26.56 ± 1.37

22.37 ± 3.35

21.85 ± 1.87*

22.85 ± 1.10*

19.26 ± 2.26*

20.23 ± 1.40*

16:1n-7

4.86 ± 0.31

5.69 ± 1.48

4.98 ± 1.74

7.28 ± 0.10*

2.36 ± 0.35*

1.84 ± 0.07*

18:0

16.90 ± 1.29

16.63 ± 2.07

15.39 ± 0.20

7.87 ± 0.48*

12.21 ± 1.18*

8.82 ± 0.31*

18:1n-9

21.97 ± 1.29

21.35 ± 3.63

25.59 ± 0.46*

25.58 ± 1.74

35.53 ± 4.81*

39.75 ± 1.89*

18:1n-7

8.47 ± 0.72

8.82 ± 1.62

4.78 ± 0.84*

4.25 ± 0.10*

3.44 ± 0.26*

2.81 ± 0.00*

18:2n-6

1.13 ± 0.13

2.63 ± 0.73

3.81 ± 0.26*

6.78 ± 0.46*

6.55 ± 0.89*

8.39 ± 0.45*

18:3n-6

0.13 ± 0.01

0.13 ± 0.01

0.25 ± 0.03*

0.27 ± 0.02*

0.30 ± 0.04*

0.31 ± 0.01*

18:3n-3

0.03 ± 0.02

0.17 ± 0.06*

0.26 ± 0.15

0.59 ± 0.02*

0.06 ± 0.04

0.13 ± 0.00*

20:0

0.26 ± 0.04

0.31 ± 0.04

0.42 ± 0.08*

0.20 ± 0.01

0.25 ± 0.02

0.13 ± 0.01*

20:4n-6

9.85 ± 0.53

9.37 ± 1.29

7.41 ± 0.30*

8.51 ± 0.39

8.31 ± 0.81

7.48 ± 0.27*

20:5n-3

0.21 ± 0.03

0.77 ± 0.24*

2.83 ± 0.59*

2.87 ± 0.16*

1.77 ± 0.09*

1.87 ± 0.08*

22:0

0.28 ± 0.01

0.27 ± 0.04

0.31 ± 0.00

0.12 ± 0.01*

0.16 ± 0.01*

0.07 ± 0.01*

22:5n-6

1.47 ± 0.07

1.29 ± 0.19

0.97 ± 0.03*

0.79 ± 0.07*

1.27 ± 0.11

1.14 ± 0.00*

22:5n-3

1.42 ± 0.05

2.46 ± 0.40*

1.46 ± 0.01

1.20 ± 0.12

1.78 ± 0.23

1.53 ± 0.03

24:0

0.35 ± 0.00

0.37 ± 0.02

0.47 ± 0.03*

0.11 ± 0.01*

0.20 ± 0.03*

0.07 ± 0.01*

22:6n-3

1.90 ± 0.06

2.47 ± 0.48

3.97 ± 0.20*

4.89 ± 0.29*

4.55 ± 0.61*

4.12 ± 0.02*

SFA

48.55 ± 3.05

44.85 ± 6.60

43.68 ± 3.46

36.98 ± 1.88*

34.08 ± 3.80*

30.63 ± 1.91*

MUFA

35.30 ± 2.32

35.86 ± 6.73

35.36 ± 3.04

37.11 ± 1.94

41.32 ± 5.41

44.41 ± 1.95*

n-6 PUFA

12.59 ± 0.74

13.42 ± 2.22

12.44 ± 0.61

16.27 ± 0.93*

16.43 ± 1.85*

17.32 ± 0.73*

n-3 PUFA

3.56 ± 0.16

5.86 ± 1.18*

8.52 ± 0.95*

9.63 ± 0.60*

8.16 ± 0.98*

7.64 ± 0.13*

Total

100.00 ± 6.27

100.00 ± 16.74

100.00 ± 8.07

100.00 ± 5.34

100.00 ± 12.04

100.00 ± 4.72

  1. HAECs were supplemented with varying doses of FO for 24 hours under standard tissue culture conditions prior to analysis. Total lipids were extracted as described in the methods. Quantification was based on the use of an internal standard and normalization to protein content. Results are expressed as the mean wt% ± SD of three determinants. *Denotes statistically significant differences (p ≤ 0.05) compared to vehicle control.
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Lipids in Health and Disease

ISSN: 1476-511X