Lipids in Health and Disease

Table 1 Primers used for quantitative real-time RT-PCR

From: Effect of testosterone deficiency on cholesterol metabolism in pigs fed a high-fat and high-cholesterol diet

Gene	Primer sequences (5′-3′)		Gen bank accession no.
LDLR	Forward	TCCAAGAGAAAGGCTCCAAG	NM_001206354.2
LDLR	Reversed	CTCCGTCACCAGCGAGTAG	NM_001206354.2
SREBP2	Forward	GATGGGCAGCAGAGTTCC	XM_005658510.1
SREBP2	Reversed	ACAGCAGCAGGTCACAGGT	XM_005658510.1
LXRα	Forward	AGATGTCCTTGTGGGTGGAG	NM_001101814.1
LXRα	Reversed	CAGAGACTGGCTGCTTGC	NM_001101814.1
HMGCR	Forward	ACAGAAGCGATGGTTGAGGT	NM_001122988.1
HMGCR	Reversed	AGATGGCAGTCACGATGTTG	NM_001122988.1
CYP7A1	Forward	GAATGACACCCTCTCCACCT	NM_001005352.2
CYP7A1	Reversed	GGAATAAGCACCAGAAAGTCG	NM_001005352.2
PCSK9	Forward	GAACCTGGAGCGGATTCTC	XM_003356453.2
PCSK9	Reversed	CACTTTGGATGCTGGTGTCT	XM_003356453.2
β-Actin	Forward	ACTGGGACGACATGGAGAAGA	DQ178122
β-Actin	Reversed	TTGGCTTTGGGGTTCAGG	DQ178122

LDLR, low-density lipoprotein receptor; SREBP2, sterol regulatory element binding protein 2; LXRα, liver X receptor alpha; HMGCR, 3-hydroxy-3-methylgutaryl coenzyme A reductase; CYP7A1, cholesterol 7α-hydroxylase; PCSK9, proprotein convertase subtilisin/kexin type 9; β-Actin, beta-actin.

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ISSN: 1476-511X

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