Effect of Lactobacillus gasseri SBT2055 (LG2055) on pancreatic lipase activity using 4-methylumbelliferyl oleate (4-MUO) as substrate. Various concentrations of LG2055 and orlistat, and 0.1 mM 4-MUO solution were mixed in the well of a microtiter plate, and the lipase solution (50 U/ml) was added. After incubation at 25°C for 30 min, 0.1 M sodium citrate (pH 4.2) was added to terminate the reaction. The suppressive activity was calculated based on the amount of 4-methylumbelliferone, in which the activity in the absence of sample was represented as 100%. Values are means with standard deviations for triplicate experiments.