Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice

Table 1 Compositions of control, high fat diet (HFD)- EPA/DHA (ED) and HFD-corn oil (CO)

Ingredient (g/100 g diet)		Control	HFD ED	HFD CO
Protein	Casein	22.2	25.6	25.6
Carbohydrates	Sucrose	5.0	10.0	10.0
	Corn starch	56.0	34.8	34.8
	Cellulose	5.0	5.8	5.8
Fat	Total	5.0	15.0	15.0
	Corn oil	2.5	3.0	5.0
	Coconut oil	2.5	10.0	10.0
	EPAX oils^a	0	2.0	0
Minerals^b		2.0	2.5	2.5
Miconutrients^c		3.0	3.0	3.0
Choline bitartrate		1.6	2.0	2.0
Cholesterol		0	1.0	1.0
Methionine		0.2	0.3	0.3
Energy content (kJ/100 g)		1599	1752	1752
	Protein E%	24	25	25
	Carbohydrate E%	65	44	44
	Fat E%	12	32	32
Fatty acid composition^d (mg/g diet)
	C10:0	0.20	1.47	1.33
	C12:0	2.37	7.58	7.72
	C14:0	1.54	4.58	4.78
	C16:0	1.90	3.44	3.59
	C18:0	0.68	2.26	2.49
	C18:1n-9	2.82	4.80	5.26
	C18:2n-6	3.62	5.03	7.36
	C18:3n-6	0.12	0.22	0.26
	C20:5n-3 (EPA)	0.00	2.03	0.01
	C22:6n-3 (DHA)	0.00	4.58	0.01
	Sum SFA	6.70	19.33	19.91
	Sum MUFA	2.82	4.80	5.26
	Sum n-6 PUFA	3.74	5.26	7.62
	Sum n-3 PUFA	0.00	6.61	0.02

^aEPAX 1050, EPAX 6015
^bCaCO₃ (57.7 %); KCl (19.9 %); KH₂PO₄ (11.9 %); MgSO₄ (10.4 %)
^cCorn starch (98.22 %); Ca(IO₃)₂ (0.0007 %); CoCO₃ (0.064 %); CuO (0.02 %); FeSO₄ (0.5 %); MnO₂ (0.035 %); Na₂MoO₄ (0.001 %); NaSeO₃ (0.0007 %); ZnO (0.1 %); Vitamin A (0.013 %); B₂ (Riboflavin-5-phosphate sodium; 0.027 %); B3 (0.1 %); B₅ (Ca Pantothenate; 0.057 %); B₆ (0.023 %); B₇ (0.0007 %); B₉ (0.007 %); B₁₂ (0.00008 %); D₃ (0.007 %); E (0.25 %); K (0.003 %)
^dAnalyses were performed in triplicates and data obtained by Gas chromatography mass spectroscopy

ISSN: 1476-511X