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Table 4 The changes in COPs contents (mg) in cholesterol with or without unsaturated fatty acids during heating at 150 °C

From: Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Treat Hour 7β-OH*** 20α-OH*** 25-OH*** Triol*** α-epoxide*** 7-keto*** Total amount of COPs
C 1 n.d.d 0.25 ± 0.01b 0.06 ± 0.00ef 0.55 ± 0.05a 0.46 ± 0.01a 1.29 ± 0.16c 2.60 ± 0.22a
  3 n.d.d n.d.l 0.10 ± 0.01bc n.d.b 0.46 ± 0.02a 1.80 ± 0.01a 2.36 ± 0.04a
  5 n.d.d 0.16 ± 0.02de 0.10 ± 0.00b n.d.b 0.25 ± 0.06b 1.19 ± 0.33cd 1.71 ± 0.42bc
  10 n.d.d 0.09 ± 0.06gh 0.10 ± 0.04b n.d.b 0.25 ± 0.08b 1.48 ± 0.11b 1.92 ± 0.30b
CL1 1 n.d.d 0.27 ± 0.01b 0.10 ± 0.02bc n.d.b 0.04 ± 0.01efgh 0.19 ± 0.02ef 0.60 ± 0.05d
(1:20) 3 n.d.d 0.11 ± 0.03fg 0.03 ± 0.01ghi n.d.b 0.10 ± 0.02cd 0.15 ± 0.04f 0.39 ± 0.09de
  5 n.d.d 0.07 ± 0.02hi 0.02 ± 0.00ghij n.d.b 0.05 ± 0.01efg 0.10 ± 0.01f 0.24 ± 0.03de
  10 2.34 ± 0.60a 0.02 ± 0.00jkl 0.004 ± 0.00J n.d.b 0.06 ± 0.02def n.d.f 2.45 ± 0.62a
CL2 1 n.d.d 0.17 ± 0.01cd 0.08 ± 0.01bcd n.d.b 0.02 ± 0.00fgh 0.12 ± 0.04f 0.40 ± 0.05de
(1:100) 3 n.d.d 0.11 ± 0.01fg 0.08 ± 0.01cde n.d.b 0.03 ± 0.00fgh 0.13 ± 0.01f 0.35 ± 0.03de
  5 n.d.D 0.05 ± 0.01ij 0.02 ± 0.00hij n.d.b 0.02 ± 0.01fgh 0.04 ± 0.01f 0.13 ± 0.03de
  10 1.40 ± 0.21b 0.01 ± 0.00kl 0.01 ± 0.00ij n.d.b 0.04 ± 0.01fgh 0.04 ± 0.02f 1.50 ± 0.25bc
CO1 1 n.d.d 0.37 ± 0.02a 0.06 ± 0.01ef n.d.b 0.02 ± 0.01fgh 0.08 ± 0.01f 0.53 ± 0.04de
(1:20) 3 n.d.d 0.13 ± 0.01ef 0.04 ± 0.01fg n.d.b 0.11 ± 0.01c n.d.f 0.29 ± 0.04de
  5 n.d.d 0.04 ± 0.00ijk 0.03 ± 0.01ghi n.d.b 0.09 ± 0.02cde n.d.f 0.16 ± 0.03de
  10 1.49 ± 0.38b 0.0003 ± 0.00l 0.009 ± 0.00ij n.d.b 0.04 ± 0.01fgh 1.06 ± 0.17d 2.60 ± 0.56a
CO2 1 n.d.d 0.20 ± 0.02c 0.07 ± 0.02de n.d.b 0.03 ± 0.01fgh 0.07 ± 0.00f 0.36 ± 0.04de
(1:100) 3 n.d.d 0.10 ± 0.01fg 0.03 ± 0.01fgh n.d.b 0.01 ± 0.01gh 0.05 ± 0.02f 0.20 ± 0.05de
  5 n.d.d 0.04 ± 0.00ijkl 0.02 ± 0.00ghij n.d.b n.d.h n.d.f 0.06 ± 0.01e
  10 0.84 ± 0.30c 0.01 ± 0.00kl 0.17 ± 0.01a n.d.b 0.02 ± 0.00fgh 0.35 ± 0.11e 1.38 ± 0.42c
  1. C cholesterol (50 mg), L linoleic acid, O: oleic acid, CL 1 cholesterol (50 mg) : linoleic acid (1 g) = 1 : 20, CL 2 cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO 1 cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO 2 cholesterol (50 mg) : oleic acid (5 g) = 1 : 100
  2. 7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 5,6α-epoxycholesterol, 7-keto 7-ketocholesterol
  3. *** P < 0.001. n.d not detected
  4. a–l Means ± SE with different superscript in the same column differ significantly