Treat
|
Hour
|
7β-OH***
|
20α-OH***
|
25-OH***
|
Triol***
|
α-epoxide***
|
7-keto***
|
Total amount of COPs
|
---|
C
|
1
|
n.d.d
|
0.25 ± 0.01b
|
0.06 ± 0.00ef
|
0.55 ± 0.05a
|
0.46 ± 0.01a
|
1.29 ± 0.16c
|
2.60 ± 0.22a
|
|
3
|
n.d.d
|
n.d.l
|
0.10 ± 0.01bc
|
n.d.b
|
0.46 ± 0.02a
|
1.80 ± 0.01a
|
2.36 ± 0.04a
|
|
5
|
n.d.d
|
0.16 ± 0.02de
|
0.10 ± 0.00b
|
n.d.b
|
0.25 ± 0.06b
|
1.19 ± 0.33cd
|
1.71 ± 0.42bc
|
|
10
|
n.d.d
|
0.09 ± 0.06gh
|
0.10 ± 0.04b
|
n.d.b
|
0.25 ± 0.08b
|
1.48 ± 0.11b
|
1.92 ± 0.30b
|
CL1
|
1
|
n.d.d
|
0.27 ± 0.01b
|
0.10 ± 0.02bc
|
n.d.b
|
0.04 ± 0.01efgh
|
0.19 ± 0.02ef
|
0.60 ± 0.05d
|
(1:20)
|
3
|
n.d.d
|
0.11 ± 0.03fg
|
0.03 ± 0.01ghi
|
n.d.b
|
0.10 ± 0.02cd
|
0.15 ± 0.04f
|
0.39 ± 0.09de
|
|
5
|
n.d.d
|
0.07 ± 0.02hi
|
0.02 ± 0.00ghij
|
n.d.b
|
0.05 ± 0.01efg
|
0.10 ± 0.01f
|
0.24 ± 0.03de
|
|
10
|
2.34 ± 0.60a
|
0.02 ± 0.00jkl
|
0.004 ± 0.00J
|
n.d.b
|
0.06 ± 0.02def
|
n.d.f
|
2.45 ± 0.62a
|
CL2
|
1
|
n.d.d
|
0.17 ± 0.01cd
|
0.08 ± 0.01bcd
|
n.d.b
|
0.02 ± 0.00fgh
|
0.12 ± 0.04f
|
0.40 ± 0.05de
|
(1:100)
|
3
|
n.d.d
|
0.11 ± 0.01fg
|
0.08 ± 0.01cde
|
n.d.b
|
0.03 ± 0.00fgh
|
0.13 ± 0.01f
|
0.35 ± 0.03de
|
|
5
|
n.d.D
|
0.05 ± 0.01ij
|
0.02 ± 0.00hij
|
n.d.b
|
0.02 ± 0.01fgh
|
0.04 ± 0.01f
|
0.13 ± 0.03de
|
|
10
|
1.40 ± 0.21b
|
0.01 ± 0.00kl
|
0.01 ± 0.00ij
|
n.d.b
|
0.04 ± 0.01fgh
|
0.04 ± 0.02f
|
1.50 ± 0.25bc
|
CO1
|
1
|
n.d.d
|
0.37 ± 0.02a
|
0.06 ± 0.01ef
|
n.d.b
|
0.02 ± 0.01fgh
|
0.08 ± 0.01f
|
0.53 ± 0.04de
|
(1:20)
|
3
|
n.d.d
|
0.13 ± 0.01ef
|
0.04 ± 0.01fg
|
n.d.b
|
0.11 ± 0.01c
|
n.d.f
|
0.29 ± 0.04de
|
|
5
|
n.d.d
|
0.04 ± 0.00ijk
|
0.03 ± 0.01ghi
|
n.d.b
|
0.09 ± 0.02cde
|
n.d.f
|
0.16 ± 0.03de
|
|
10
|
1.49 ± 0.38b
|
0.0003 ± 0.00l
|
0.009 ± 0.00ij
|
n.d.b
|
0.04 ± 0.01fgh
|
1.06 ± 0.17d
|
2.60 ± 0.56a
|
CO2
|
1
|
n.d.d
|
0.20 ± 0.02c
|
0.07 ± 0.02de
|
n.d.b
|
0.03 ± 0.01fgh
|
0.07 ± 0.00f
|
0.36 ± 0.04de
|
(1:100)
|
3
|
n.d.d
|
0.10 ± 0.01fg
|
0.03 ± 0.01fgh
|
n.d.b
|
0.01 ± 0.01gh
|
0.05 ± 0.02f
|
0.20 ± 0.05de
|
|
5
|
n.d.d
|
0.04 ± 0.00ijkl
|
0.02 ± 0.00ghij
|
n.d.b
|
n.d.h
|
n.d.f
|
0.06 ± 0.01e
|
|
10
|
0.84 ± 0.30c
|
0.01 ± 0.00kl
|
0.17 ± 0.01a
|
n.d.b
|
0.02 ± 0.00fgh
|
0.35 ± 0.11e
|
1.38 ± 0.42c
|
-
C cholesterol (50 mg), L linoleic acid, O: oleic acid, CL
1
cholesterol (50 mg) : linoleic acid (1 g) = 1 : 20, CL
2
cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO
1
cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO
2
cholesterol (50 mg) : oleic acid (5 g) = 1 : 100
-
7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 5,6α-epoxycholesterol, 7-keto 7-ketocholesterol
-
***
P < 0.001. n.d not detected
-
a–l Means ± SE with different superscript in the same column differ significantly