Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Min, Joong-Seok; Lee, Sang-Ok; Khan, Muhammad Issa; Yim, Dong Gyun; Seol, Kuk-Hwan; Lee, Mooha; Jo, Cheorun

doi:10.1186/s12944-015-0074-6

Lipids in Health and Disease

Table 4 The changes in COPs contents (mg) in cholesterol with or without unsaturated fatty acids during heating at 150 °C

From: Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Treat	Hour	7β-OH^***	20α-OH^***	25-OH^***	Triol^***	α-epoxide^***	7-keto^***	Total amount of COPs
C	1	n.d.^d	0.25 ± 0.01^b	0.06 ± 0.00^ef	0.55 ± 0.05^a	0.46 ± 0.01^a	1.29 ± 0.16^c	2.60 ± 0.22^a
	3	n.d.^d	n.d.^l	0.10 ± 0.01^bc	n.d.^b	0.46 ± 0.02^a	1.80 ± 0.01^a	2.36 ± 0.04^a
	5	n.d.^d	0.16 ± 0.02^de	0.10 ± 0.00^b	n.d.^b	0.25 ± 0.06^b	1.19 ± 0.33^cd	1.71 ± 0.42^bc
	10	n.d.^d	0.09 ± 0.06^gh	0.10 ± 0.04^b	n.d.^b	0.25 ± 0.08^b	1.48 ± 0.11^b	1.92 ± 0.30^b
CL₁	1	n.d.^d	0.27 ± 0.01^b	0.10 ± 0.02^bc	n.d.^b	0.04 ± 0.01^efgh	0.19 ± 0.02^ef	0.60 ± 0.05^d
(1:20)	3	n.d.^d	0.11 ± 0.03^fg	0.03 ± 0.01^ghi	n.d.^b	0.10 ± 0.02^cd	0.15 ± 0.04^f	0.39 ± 0.09^de
	5	n.d.^d	0.07 ± 0.02^hi	0.02 ± 0.00^ghij	n.d.^b	0.05 ± 0.01^efg	0.10 ± 0.01^f	0.24 ± 0.03^de
	10	2.34 ± 0.60^a	0.02 ± 0.00^jkl	0.004 ± 0.00^J	n.d.^b	0.06 ± 0.02^def	n.d.^f	2.45 ± 0.62^a
CL₂	1	n.d.^d	0.17 ± 0.01^cd	0.08 ± 0.01^bcd	n.d.^b	0.02 ± 0.00^fgh	0.12 ± 0.04^f	0.40 ± 0.05^de
(1:100)	3	n.d.^d	0.11 ± 0.01^fg	0.08 ± 0.01^cde	n.d.^b	0.03 ± 0.00^fgh	0.13 ± 0.01^f	0.35 ± 0.03^de
	5	n.d.^D	0.05 ± 0.01^ij	0.02 ± 0.00^hij	n.d.^b	0.02 ± 0.01^fgh	0.04 ± 0.01^f	0.13 ± 0.03^de
	10	1.40 ± 0.21^b	0.01 ± 0.00^kl	0.01 ± 0.00^ij	n.d.^b	0.04 ± 0.01^fgh	0.04 ± 0.02^f	1.50 ± 0.25^bc
CO₁	1	n.d.^d	0.37 ± 0.02^a	0.06 ± 0.01^ef	n.d.^b	0.02 ± 0.01^fgh	0.08 ± 0.01^f	0.53 ± 0.04^de
(1:20)	3	n.d.^d	0.13 ± 0.01^ef	0.04 ± 0.01^fg	n.d.^b	0.11 ± 0.01^c	n.d.^f	0.29 ± 0.04^de
	5	n.d.^d	0.04 ± 0.00^ijk	0.03 ± 0.01^ghi	n.d.^b	0.09 ± 0.02^cde	n.d.^f	0.16 ± 0.03^de
	10	1.49 ± 0.38^b	0.0003 ± 0.00^l	0.009 ± 0.00^ij	n.d.^b	0.04 ± 0.01^fgh	1.06 ± 0.17^d	2.60 ± 0.56^a
CO₂	1	n.d.^d	0.20 ± 0.02^c	0.07 ± 0.02^de	n.d.^b	0.03 ± 0.01^fgh	0.07 ± 0.00^f	0.36 ± 0.04^de
(1:100)	3	n.d.^d	0.10 ± 0.01^fg	0.03 ± 0.01^fgh	n.d.^b	0.01 ± 0.01^gh	0.05 ± 0.02^f	0.20 ± 0.05^de
	5	n.d.^d	0.04 ± 0.00^ijkl	0.02 ± 0.00^ghij	n.d.^b	n.d.^h	n.d.^f	0.06 ± 0.01^e
	10	0.84 ± 0.30^c	0.01 ± 0.00^kl	0.17 ± 0.01^a	n.d.^b	0.02 ± 0.00^fgh	0.35 ± 0.11^e	1.38 ± 0.42^c

C cholesterol (50 mg), L linoleic acid, O: oleic acid, CL ₁ cholesterol (50 mg) : linoleic acid (1 g) = 1 : 20, CL ₂ cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO ₁ cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO ₂ cholesterol (50 mg) : oleic acid (5 g) = 1 : 100
7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 5,6α-epoxycholesterol, 7-keto 7-ketocholesterol
^*** P < 0.001. n.d not detected
^a–l Means ± SE with different superscript in the same column differ significantly

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