Treat
|
Hour
|
7β-OH***
|
20α-OH***
|
25-OH***
|
Triol***
|
α-epoxide***
|
7-keto***
|
Total amount of COPs
|
---|
C
|
1
|
n.d.e
|
0.18 ± 0.01a
|
0.10 ± 0.02bc
|
n.d.c
|
0.17 ± 0.01a
|
0.88 ± 0.12bc
|
1.33 ± 0.16def
|
|
3
|
n.d.e
|
0.18 ± 0.01a
|
0.09 ± 0.01cd
|
n.d.c
|
0.11 ± 0.02bc
|
0.94 ± 0.17b
|
1.32 ± 0.21def
|
|
5
|
n.d.e
|
0.08 ± 0.03b
|
0.08 ± 0.01e
|
n.d.c
|
0.12 ± 0.03b
|
1.10 ± 0.11a
|
1.38 ± 0.17def
|
|
10
|
n.d.e
|
0.03 ± 0.01e
|
0.06 ± 0.01e
|
n.d.c
|
0.09 ± 0.03bcd
|
0.82 ± 0.08bc
|
1.00 ± 0.12efg
|
CL1
|
1
|
5.75 ± 0.40b
|
0.05 ± 0.01c
|
0.02 ± 0.00fg
|
n.d.c
|
0.08 ± 0.04cdef
|
0.09 ± 0.03ef
|
5.99 ± 0.48b
|
(1:20)
|
3
|
0.55 ± 0.02de
|
0.002 ± 0.00F
|
0.08 ± 0.02de
|
n.d.c
|
0.10 ± 0.06bc
|
0.10 ± 0.02ef
|
0.83 ± 0.12efgh
|
|
5
|
0.24 ± 0.02de
|
n.d.f
|
0.10 ± 0.01bc
|
n.d.c
|
0.18 ± 0.02a
|
0.20 ± 0.09e
|
0.72 ± 0.14fgh
|
|
10
|
0.18 ± 0.04de
|
n.d.f
|
0.11 ± 0.01ab
|
0.02 ± 0.01b
|
0.12 ± 0.02b
|
0.11 ± 0.01ef
|
0.54 ± 0.08gh
|
CL2
|
1
|
3.33 ± 0.67c
|
0.04 ± 0.00De
|
0.02 ± 0.00fg
|
n.d.c
|
0.02 ± 0.01gh
|
n.d.f
|
3.41 ± 0.68c
|
(1:100)
|
3
|
0.19 ± 0.02de
|
0.002 ± 0.00f
|
0.01 ± 0.00gh
|
0.02 ± 0.01b
|
0.03 ± 00.01gh
|
0.03 ± 0.01f
|
0.28 ± 0.04h
|
|
5
|
0.40 ± 0.08de
|
0.002 ± 0.00f
|
0.03 ± 0.00fg
|
0.03 ± 0.00a
|
0.06 ± 0.00defg
|
0.04 ± 0.00f
|
0.56 ± 0.09gh
|
|
10
|
0.29 ± 0.02de
|
n.d.f
|
0.03 ± 0.01f
|
0.03 ± 0.00a
|
0.08 ± 0.01cde
|
n.d.f
|
0.44 ± 0.04gh
|
CO1
|
1
|
6.51 ± 0.97a
|
0.05 ± 0.01cd
|
0.06 ± 0.01e
|
n.d.c
|
0.04 ± 0.01gh
|
0.77 ± 0.08c
|
7.43 ± 1.08a
|
(1:20)
|
3
|
0.65 ± 0.02d
|
n.d.f
|
0.13 ± 0.01a
|
n.d.c
|
0.04 ± 0.01fgh
|
0.90 ± 0.13bc
|
1.71 ± 0.17d
|
|
5
|
0.49 ± 0.09de
|
0.001 ± 0.00f
|
0.04 ± 0.01f
|
n.d.c
|
0.02 ± 0.00gh
|
n.d.f
|
0.54 ± 0.11gh
|
|
10
|
0.21 ± 0.03de
|
n.d.f
|
n.d.h
|
n.d.c
|
0.01 ± 0.00h
|
n.d.f
|
0.22 ± 0.03h
|
CO2
|
1
|
3.08 ± 0.33c
|
0.04 ± 0.01cde
|
0.03 ± 0.01f
|
n.d.c
|
0.02 ± 0.01gh
|
n.d.f
|
3.18 ± 0.35c
|
(1:100)
|
3
|
0.24 ± 0.02de
|
n.d.f
|
0.06 ± 0.01e
|
n.d.c
|
0.02 ± 0.01gh
|
0.33 ± 0.06d
|
0.65 ± 0.10gh
|
|
5
|
0.13 ± 0.03de
|
n.d.f
|
n.d.h
|
n.d.c
|
0.05 ± 0.01efgh
|
0.37 ± 0.02d
|
0.55 ± 0.06gh
|
|
10
|
0.14 ± 0.03de
|
n.d.f
|
n.d.h
|
n.d.c
|
0.03 ± 0.01gh
|
0.42 ± 0.10d
|
0.58 ± 0.13gh
|
-
C cholesterol (50 mg), L linoleic acid, O oleic acid, CL
1
cholesterol (50 mg): linoleic acid (1 g) = 1 : 20, CL
2
cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO1: cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO
2
cholesterol (50 mg): oleic acid (5 g) = 1 : 100
-
7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 56α-epoxycholesterol, 7-keto 7-ketocholesterol
-
***
P < 0.001, n.d not detected
-
a–h Means ± SE with different superscript in the same column differ significantly