Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Min, Joong-Seok; Lee, Sang-Ok; Khan, Muhammad Issa; Yim, Dong Gyun; Seol, Kuk-Hwan; Lee, Mooha; Jo, Cheorun

doi:10.1186/s12944-015-0074-6

Lipids in Health and Disease

Table 5 The changes in COPs contents (mg) in cholesterol with or without unsaturated fatty acids during heating at 200 °C

From: Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Treat	Hour	7β-OH^***	20α-OH^***	25-OH^***	Triol^***	α-epoxide^***	7-keto^***	Total amount of COPs
C	1	n.d.^e	0.18 ± 0.01^a	0.10 ± 0.02^bc	n.d.^c	0.17 ± 0.01^a	0.88 ± 0.12^bc	1.33 ± 0.16^def
	3	n.d.^e	0.18 ± 0.01^a	0.09 ± 0.01^cd	n.d.^c	0.11 ± 0.02^bc	0.94 ± 0.17^b	1.32 ± 0.21^def
	5	n.d.^e	0.08 ± 0.03^b	0.08 ± 0.01^e	n.d.^c	0.12 ± 0.03^b	1.10 ± 0.11^a	1.38 ± 0.17^def
	10	n.d.^e	0.03 ± 0.01^e	0.06 ± 0.01^e	n.d.^c	0.09 ± 0.03^bcd	0.82 ± 0.08^bc	1.00 ± 0.12^efg
CL₁	1	5.75 ± 0.40^b	0.05 ± 0.01^c	0.02 ± 0.00^fg	n.d.^c	0.08 ± 0.04^cdef	0.09 ± 0.03^ef	5.99 ± 0.48^b
(1:20)	3	0.55 ± 0.02^de	0.002 ± 0.00^F	0.08 ± 0.02^de	n.d.^c	0.10 ± 0.06^bc	0.10 ± 0.02^ef	0.83 ± 0.12^efgh
	5	0.24 ± 0.02^de	n.d.^f	0.10 ± 0.01^bc	n.d.^c	0.18 ± 0.02^a	0.20 ± 0.09^e	0.72 ± 0.14^fgh
	10	0.18 ± 0.04^de	n.d.^f	0.11 ± 0.01^ab	0.02 ± 0.01^b	0.12 ± 0.02^b	0.11 ± 0.01^ef	0.54 ± 0.08^gh
CL₂	1	3.33 ± 0.67^c	0.04 ± 0.00D^e	0.02 ± 0.00^fg	n.d.^c	0.02 ± 0.01^gh	n.d.^f	3.41 ± 0.68^c
(1:100)	3	0.19 ± 0.02^de	0.002 ± 0.00^f	0.01 ± 0.00^gh	0.02 ± 0.01^b	0.03 ± 00.01^gh	0.03 ± 0.01^f	0.28 ± 0.04^h
	5	0.40 ± 0.08^de	0.002 ± 0.00^f	0.03 ± 0.00^fg	0.03 ± 0.00^a	0.06 ± 0.00^defg	0.04 ± 0.00^f	0.56 ± 0.09^gh
	10	0.29 ± 0.02^de	n.d.^f	0.03 ± 0.01^f	0.03 ± 0.00^a	0.08 ± 0.01^cde	n.d.^f	0.44 ± 0.04^gh
CO₁	1	6.51 ± 0.97^a	0.05 ± 0.01^cd	0.06 ± 0.01^e	n.d.^c	0.04 ± 0.01^gh	0.77 ± 0.08^c	7.43 ± 1.08^a
(1:20)	3	0.65 ± 0.02^d	n.d.^f	0.13 ± 0.01^a	n.d.^c	0.04 ± 0.01^fgh	0.90 ± 0.13^bc	1.71 ± 0.17^d
	5	0.49 ± 0.09^de	0.001 ± 0.00^f	0.04 ± 0.01^f	n.d.^c	0.02 ± 0.00^gh	n.d.^f	0.54 ± 0.11^gh
	10	0.21 ± 0.03^de	n.d.^f	n.d.^h	n.d.^c	0.01 ± 0.00^h	n.d.^f	0.22 ± 0.03^h
CO₂	1	3.08 ± 0.33^c	0.04 ± 0.01^cde	0.03 ± 0.01^f	n.d.^c	0.02 ± 0.01^gh	n.d.^f	3.18 ± 0.35^c
(1:100)	3	0.24 ± 0.02^de	n.d.^f	0.06 ± 0.01^e	n.d.^c	0.02 ± 0.01^gh	0.33 ± 0.06^d	0.65 ± 0.10^gh
	5	0.13 ± 0.03^de	n.d.^f	n.d.^h	n.d.^c	0.05 ± 0.01^efgh	0.37 ± 0.02^d	0.55 ± 0.06^gh
	10	0.14 ± 0.03^de	n.d.^f	n.d.^h	n.d.^c	0.03 ± 0.01^gh	0.42 ± 0.10^d	0.58 ± 0.13^gh

C cholesterol (50 mg), L linoleic acid, O oleic acid, CL ₁ cholesterol (50 mg): linoleic acid (1 g) = 1 : 20, CL ₂ cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO₁: cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO ₂ cholesterol (50 mg): oleic acid (5 g) = 1 : 100
7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 56α-epoxycholesterol, 7-keto 7-ketocholesterol
^*** P < 0.001, n.d not detected
^a–h Means ± SE with different superscript in the same column differ significantly

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