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Table 5 The changes in COPs contents (mg) in cholesterol with or without unsaturated fatty acids during heating at 200 °C

From: Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Treat Hour 7β-OH*** 20α-OH*** 25-OH*** Triol*** α-epoxide*** 7-keto*** Total amount of COPs
C 1 n.d.e 0.18 ± 0.01a 0.10 ± 0.02bc n.d.c 0.17 ± 0.01a 0.88 ± 0.12bc 1.33 ± 0.16def
  3 n.d.e 0.18 ± 0.01a 0.09 ± 0.01cd n.d.c 0.11 ± 0.02bc 0.94 ± 0.17b 1.32 ± 0.21def
  5 n.d.e 0.08 ± 0.03b 0.08 ± 0.01e n.d.c 0.12 ± 0.03b 1.10 ± 0.11a 1.38 ± 0.17def
  10 n.d.e 0.03 ± 0.01e 0.06 ± 0.01e n.d.c 0.09 ± 0.03bcd 0.82 ± 0.08bc 1.00 ± 0.12efg
CL1 1 5.75 ± 0.40b 0.05 ± 0.01c 0.02 ± 0.00fg n.d.c 0.08 ± 0.04cdef 0.09 ± 0.03ef 5.99 ± 0.48b
(1:20) 3 0.55 ± 0.02de 0.002 ± 0.00F 0.08 ± 0.02de n.d.c 0.10 ± 0.06bc 0.10 ± 0.02ef 0.83 ± 0.12efgh
  5 0.24 ± 0.02de n.d.f 0.10 ± 0.01bc n.d.c 0.18 ± 0.02a 0.20 ± 0.09e 0.72 ± 0.14fgh
  10 0.18 ± 0.04de n.d.f 0.11 ± 0.01ab 0.02 ± 0.01b 0.12 ± 0.02b 0.11 ± 0.01ef 0.54 ± 0.08gh
CL2 1 3.33 ± 0.67c 0.04 ± 0.00De 0.02 ± 0.00fg n.d.c 0.02 ± 0.01gh n.d.f 3.41 ± 0.68c
(1:100) 3 0.19 ± 0.02de 0.002 ± 0.00f 0.01 ± 0.00gh 0.02 ± 0.01b 0.03 ± 00.01gh 0.03 ± 0.01f 0.28 ± 0.04h
  5 0.40 ± 0.08de 0.002 ± 0.00f 0.03 ± 0.00fg 0.03 ± 0.00a 0.06 ± 0.00defg 0.04 ± 0.00f 0.56 ± 0.09gh
  10 0.29 ± 0.02de n.d.f 0.03 ± 0.01f 0.03 ± 0.00a 0.08 ± 0.01cde n.d.f 0.44 ± 0.04gh
CO1 1 6.51 ± 0.97a 0.05 ± 0.01cd 0.06 ± 0.01e n.d.c 0.04 ± 0.01gh 0.77 ± 0.08c 7.43 ± 1.08a
(1:20) 3 0.65 ± 0.02d n.d.f 0.13 ± 0.01a n.d.c 0.04 ± 0.01fgh 0.90 ± 0.13bc 1.71 ± 0.17d
  5 0.49 ± 0.09de 0.001 ± 0.00f 0.04 ± 0.01f n.d.c 0.02 ± 0.00gh n.d.f 0.54 ± 0.11gh
  10 0.21 ± 0.03de n.d.f n.d.h n.d.c 0.01 ± 0.00h n.d.f 0.22 ± 0.03h
CO2 1 3.08 ± 0.33c 0.04 ± 0.01cde 0.03 ± 0.01f n.d.c 0.02 ± 0.01gh n.d.f 3.18 ± 0.35c
(1:100) 3 0.24 ± 0.02de n.d.f 0.06 ± 0.01e n.d.c 0.02 ± 0.01gh 0.33 ± 0.06d 0.65 ± 0.10gh
  5 0.13 ± 0.03de n.d.f n.d.h n.d.c 0.05 ± 0.01efgh 0.37 ± 0.02d 0.55 ± 0.06gh
  10 0.14 ± 0.03de n.d.f n.d.h n.d.c 0.03 ± 0.01gh 0.42 ± 0.10d 0.58 ± 0.13gh
  1. C cholesterol (50 mg), L linoleic acid, O oleic acid, CL 1 cholesterol (50 mg): linoleic acid (1 g) = 1 : 20, CL 2 cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO1: cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO 2 cholesterol (50 mg): oleic acid (5 g) = 1 : 100
  2. 7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 56α-epoxycholesterol, 7-keto 7-ketocholesterol
  3. *** P < 0.001, n.d not detected
  4. a–h Means ± SE with different superscript in the same column differ significantly