Fig. 5From: Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cellsPalmitate induces phospholipid accumulation in RAW 264.7 cells. Cells were treated with 400 μM palmitate or 10 μg/ml tunicamycin for 24 h and then subjected to immunofluorescence using calnexin antibody and LipidTOX Red dye to examine phospholipid accumulation in ER. Green fluorescence indicates calnexin or ER membrane, red fluorescence indicates phospholipids and yellow fluorescence indicates co-localization of phospholipids with ER membraneBack to article page