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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cells

Fig. 7

Palmitate-induced phospholipid accumulation is inhibited by the down-regulation of XBP-1 or CCTα in RAW 264.7 cells. Cells were transfected with control, XBP-1 or CCTα siRNAs. After 48 h transfection, the effect of XBP-1 or CCTα siRNA transfection was confirmed by western blot analysis (a). The transfected cells were treated with 400 μM palmitate for 24 h and then subjected to immunofluorescence using calnexin antibody and LipidTOX Red dye to examine phospholipid accumulation in ER. Green fluorescence indicates calnexin or ER membrane, red fluorescence indicates phospholipids and yellow fluorescence indicates co-localization of phospholipids with ER membrane (b)

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Lipids in Health and Disease

ISSN: 1476-511X