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Fig. 9 | Lipids in Health and Disease

Fig. 9

From: Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cells

Fig. 9

Tunicamycin does not induces cisternal ER expansion in RAW 264.7 cells. Cells were treated with 10 μg/ml tunicamycin for 24 h. The phosphorylation of PERK, and the expression of BiP and CHOP were determined by western blot analysis (a). Total RNA was extracted and reverse transcribed to cDNA. Full-length and spliced XBP-1 cDNAs were amplified by PCR with mouse XBP-1 primers. Gapdh cDNA was included as an internal loading control (b). Changes in the ER structure were examined by transmission electron microscopy (c)

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Lipids in Health and Disease

ISSN: 1476-511X