Fig. 5

MALDI-IMS-MS images mapping the ions m/z 759.56, 757.54, 732.54 and 703.56 in skin equivalent tissue, at the different stages of a time course (4, 6 and 24 h respectively). a - (c) m/z 732.54 at 4,6, and 24 h, (d)-(f) m/z 757.54 (g)-(i) m/z 759.56 at 4,6 and 24 h and (j)-(l) m/z 703.56 at 4,6, and 24 h . These signals were identified from the PCA data as being significant contributors of change in the spectra between the epidermis and dermis. Images are at a spatial resolution of 50 μm × 50 μm and are normalised against the total ion count. Optical images of Oil Red O stained sections of the tissue at the different stages of a time course (4, 6 and 24 h respectively) are also shown (m)-(o)