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Fig. 6 | Lipids in Health and Disease

Fig. 6

From: Aerobic exercise training enhances the in vivo cholesterol trafficking from macrophages to the liver independently of changes in the expression of genes involved in lipid flux in macrophages and aorta

Fig. 6

Cyp7a1 and Cy27a1 mRNA expression in the liver samples of trained and sedentary C57BL/6N wild type mice by quantitative real-time PCR. Using reverse transcriptase, cDNA was synthetized from 2 μg from total RNA isolated from the liver samples of trained (n = 6 - black bars) and sedentary (n = 6 - white bars). The TaqMan gene expression assays used were Mm00484150_m1 (Cyp7a1) and Mm00470430_m1 (Cyp27a1) and quantification was normalized to the endogenous Actb (Mm00607939_s1). Real-time PCR was performed using Gene Expression Master Mix (Applied Biosystems). Data analysis was performed using 2-ΔΔCt method. Data are expressed as mean values ± standard error

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Lipids in Health and Disease

ISSN: 1476-511X