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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: Aerobic exercise training enhances the in vivo cholesterol trafficking from macrophages to the liver independently of changes in the expression of genes involved in lipid flux in macrophages and aorta

Fig. 7

Expression of genes involved in lipid flux in macrophages. Peritoneal macrophages were harvested from trained (n = 6 - black bars) and sedentary (n = 6 - white bars) C57BL/6N wild type animals immediately (0 h panel a) and 48 h (panel b) after the last exercise session. Macrophages were ressuspendend in Trizol and gene expression was determined by quantitative real-time PCR. Using reverse transcriptase, cDNA was synthetized from 100 ng from total RNA isolated from macrophages of trained (black bars) and sedentary (white bars). The TaqMan gene expression assays used were: Cd36 - Mm01135198_m1, Olr1 Mm00454586_m1, Scarb1 - Mm00450234_m1, Pparg - Mm01184322_m1, Nr1h3 Mm01329744_g1, Nr1h2 - Mm00437265_g1, Abca1 - Mm00442646_m1, Abcg1 Mm00437390_m1 and quantification was normalized to the endogenous Actb (Mm00607939_s1). Real-time PCR was performed using Gene Expression Master Mix (Applied Biosystems). Data analysis was performed using 2-ΔΔCt method. Data are expressed as mean values ± standard error

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Lipids in Health and Disease

ISSN: 1476-511X