Joint associations of folate, homocysteine and MTHFR, MTR and MTRR gene polymorphisms with dyslipidemia in a Chinese hypertensive population: a cross-sectional study

Table 8 Primer sequences and reaction conditions for PCR-RFLP of gene polymorphisms

Gene	Primer sequence	T^a and Cycles	Product size	Restriction enzyme
MTHFR C677T	Forward: 5’-TGAAGGAGAAGGTGTCTGCGGGA-3’	58 °C, 35	198 bp	Hinf I
MTHFR C677T	Reverse: 5’-AGGACGGTGCGGTGAGAGTG-3’	58 °C, 35	198 bp	Hinf I
MTHFR A1298C	Forward: 5’-CTTTGGGGAGCTGAAGGACTACTAC-3’	52 °C, 38	163 bp	Mbo II
MTHFR A1298C	Reverse: 5’-CACTTTGTGACCATTCCGGTTTG-3’	52 °C, 38	163 bp	Mbo II
MTR A2756G	Forward: 5’-GAACTAGAAGACAGAAATTCTCTA-3’	53 °C, 36	189 bp	Hae III
MTR A2756G	Reverse: 5’-CATGGAAGAATATCAAGATATTAGA-3’	53 °C, 36	189 bp	Hae III
MTRR A66G	Forward: 5’-GCAAAGGCCATCGCAGAAGACAT-3’	60 °C, 35	151 bp	Nsp I
MTRR A66G	Reverse: 5’-GTGAAGATCTGCAGAAAATCCATGTA-3’	60 °C, 35	151 bp	Nsp I

ISSN: 1476-511X