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Table 8 Primer sequences and reaction conditions for PCR-RFLP of gene polymorphisms

From: Joint associations of folate, homocysteine and MTHFR, MTR and MTRR gene polymorphisms with dyslipidemia in a Chinese hypertensive population: a cross-sectional study

Gene Primer sequence Ta and Cycles Product size Restriction enzyme
MTHFR C677T Forward: 5’-TGAAGGAGAAGGTGTCTGCGGGA-3’ 58 °C, 35 198 bp Hinf I
Reverse: 5’-AGGACGGTGCGGTGAGAGTG-3’
MTHFR A1298C Forward: 5’-CTTTGGGGAGCTGAAGGACTACTAC-3’ 52 °C, 38 163 bp Mbo II
Reverse: 5’-CACTTTGTGACCATTCCGGTTTG-3’
MTR A2756G Forward: 5’-GAACTAGAAGACAGAAATTCTCTA-3’ 53 °C, 36 189 bp Hae III
Reverse: 5’-CATGGAAGAATATCAAGATATTAGA-3’
MTRR A66G Forward: 5’-GCAAAGGCCATCGCAGAAGACAT-3’ 60 °C, 35 151 bp Nsp I
Reverse: 5’-GTGAAGATCTGCAGAAAATCCATGTA-3’
  1. aAnnealing temperature