Table 5 Sequence of the primers, enzymes and sizes of PCR-RFLP fragments
Polymorphisms | Primer sequence | Annealing temp. (°C) | Product size (bp) | Restriction enzyme/allele sizes | ||
|---|---|---|---|---|---|---|
GEN LEPR | ||||||
BstUI | ||||||
1968 G/C (rs8179183) | F | 5′-AATCCAGCCTACACAGTTGT-3′ | 63 | 224 | G/G | 224 |
R | 5′-CTTCCAAAGTAAAGTGACATTTTTCGC-3′ | G/C | 224, 198, 26 | |||
C/C | 198, 26 | |||||
MspI | ||||||
668 A/G (rs1137101) | F | 5′-AAACTCAA CGACACTCTC CTT-3′ | 63 | 80 | A/A | 80 |
R | 5′-TGAACTGACATTAGAGGTGA-3′ | A/G | 80, 57, 23 | |||
G/G | 57, 23 | |||||
GEN APOA5 | ||||||
Sau96I | ||||||
56 C/G (rs3135506) | F | 5′-CACAGAGGTTGAGGCAGCAGA-3′ | 60 | 335 | C/C | 151, 77, 40, 35, 31, 1 |
R | 5′-GGCTCTGGCTCTTCTTTCAGG-3′ | C/G | 186, 151, 77, 40, 35, 31, 1 | |||
G/G | 186, 77, 40, 31, 1 | |||||
MseI | ||||||
−1131 T/C (rs662799) | F | 5′-GGAGC TTGTGAACGTGTGTATGAGT-3′ | 58 | 187 | T/T | 157, 30 |
R | 5′-CCCCAGGAAC TGG AGC GAA ATT-3′ | T/C | 187, 157, 30 | |||
C/C | 187 | |||||
GEN APOA2 | ||||||
SmlI | ||||||
−265 T/C (rs5082) | F | 5′-GATAAGGTTGAGAGATGAGATCT-3′ | 62 | 310 | T/T | 310 |
R | 5′-GTGAGGATAAACAAGTTGGAGAA-3′ | T/C | 310,167,143 | |||
C/C | 167,143 | |||||
NheI | ||||||
−1730 G/T (rs3813627) | F | 5′-GAATAGTTCTGCTAGCACTTAC-3′ | 64 | 162 | G/G | 151, 11 |
R | 5′-CAAATGAGTACTACTCATTAAG-3′ | G/T | 162, 151, 11 | |||
T/T | 162 | |||||