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Table 5 Sequence of the primers, enzymes and sizes of PCR-RFLP fragments

From: Interaction of dietary fat intake with APOA2, APOA5 and LEPR polymorphisms and its relationship with obesity and dyslipidemia in young subjects

Polymorphisms Primer sequence Annealing temp. (°C) Product size (bp) Restriction enzyme/allele sizes
GEN LEPR
       BstUI
1968 G/C (rs8179183) F 5′-AATCCAGCCTACACAGTTGT-3′ 63 224 G/G 224
R 5′-CTTCCAAAGTAAAGTGACATTTTTCGC-3′ G/C 224, 198, 26
C/C 198, 26
       MspI
668 A/G (rs1137101) F 5′-AAACTCAA CGACACTCTC CTT-3′ 63 80 A/A 80
R 5′-TGAACTGACATTAGAGGTGA-3′ A/G 80, 57, 23
G/G 57, 23
GEN APOA5
       Sau96I
56 C/G (rs3135506) F 5′-CACAGAGGTTGAGGCAGCAGA-3′ 60 335 C/C 151, 77, 40, 35, 31, 1
R 5′-GGCTCTGGCTCTTCTTTCAGG-3′ C/G 186, 151, 77, 40, 35, 31, 1
G/G 186, 77, 40, 31, 1
       MseI
−1131 T/C (rs662799) F 5′-GGAGC TTGTGAACGTGTGTATGAGT-3′ 58 187 T/T 157, 30
R 5′-CCCCAGGAAC TGG AGC GAA ATT-3′ T/C 187, 157, 30
C/C 187
GEN APOA2
       SmlI
−265 T/C (rs5082) F 5′-GATAAGGTTGAGAGATGAGATCT-3′ 62 310 T/T 310
R 5′-GTGAGGATAAACAAGTTGGAGAA-3′ T/C 310,167,143
C/C 167,143
     NheI
−1730 G/T (rs3813627) F 5′-GAATAGTTCTGCTAGCACTTAC-3′ 64 162 G/G 151, 11
R 5′-CAAATGAGTACTACTCATTAAG-3′ G/T 162, 151, 11
T/T 162