Fig. 1

ApoJ overexpression by infection with recombinant adenovirus prevents AngII-induced apoptosis in NRVCs. a Apo J expression after infection by recombinant adenovirus was determined by Western blot (n = 3/group). b Cleavage of caspase-3 was stimulated by AngII and inhibited by ApoJ (n = 3/group). c Control and ApoJ-transduced NRVCs were incubated with AngII for 24 h, and then cell viability was determined by MTT assay (n = 8/group). d Apoptosis of cardiomyocytes was determined by caspase-3/7 activity assay. Apoptosis of control adenovirus infected NRVCs was significantly increased, while ApoJ-overexpressing NRVCs showed resistance to AngII-induced cell injury (n = 8/group). e LY294002, Mn(III)TBAP, SB203580 and PDTC were pretreated in NRVCs for 1 h, and then the cells were incubated with AngII for 24 h. Cell apoptosis was determined by caspase-3/7 activity assay (n = 8/group). **P < 0.01 versus control Ad/PBS group; ^## P < 0.01 versus control Ad/AngII group, ^$ P < 0.05 versus control Ad/AngII group