Table 2 Oligonucleotide primers used for quantitative real-time polymerase chain reaction
Gene | Oligo FWD | Oligo REV |
|---|---|---|
MDR2 | ggcattctccatcatcctgt | cacttctgttgctttactgtgtca |
BSEP | cggtggctgagagatcaaat | tgcgatagtggtggagaaca |
ABCG5 | cggagagttggtgttctgtg | caccgatgtcaagtccatgt |
ABCG8 | cagatgctggctatcataggg | ctgatttcatcttgccacca |
ACAT-2 | cctcacagatgcgtttcaca | ctctgctcacttgccattttt |
Apob | gatggagatgggagatgaggt | gggctcctcatcaacaagag |
Cideb | gctccaatggcctgctaag | ttatgatcacagacacggaagg |
Cyp7a1 | ggagcttatttcaaatgatcagg | cactctgtaaagctccactcactt |
DGAT-2 | aggatctgccctgtcacg | gtcttggagggccgagag |
HMG-CoAr | caaccttctacctcagcaagc | acagtgccacacacaattcg |
LDLr | tgctactggccaaggacat | ctgggtggtcggtacagtg |
LRP-1 | aatcgagggcaagatgacac | ccagtctgtccagtacatccac |
Mttp | gcgagtctaaaacccgagtg | cactgtgatgtcgctggttatt |
FXR | ccacgaccaagctatgcag | tctctgtttgctgtatgagtcca |
Sar1a | gggcaaaccacaggaaag | cactgcacatgaacacttcca |
SREBP-2 | gtgcagacagtcgctacacc | aatctgaggctgaaccagga |
NTCP | aaaatcaagcctccaaaggac | ttgtgggtacctttttccaga |
ActB | cccgcgagtacaaccttct | cgtcatccatggcgaact |
GAPDH | ccctcaagattgtcagcaatg | agttgtcatggatgaccttgg |