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Table 1 Changes of neutral SMase activity (pmol/h/mg protein) after mevalonate treatment in Caco-2 and HepG2 cells

From: Mevalonate inhibits acid sphingomyelinase activity, increases sphingomyelin levels and inhibits cell proliferation of HepG2 and Caco-2 cells

Cell line

Time (h)

Mevalonate (mM)

0

5

10

20

Caco-2

24

100 ± 11.9

103.1 ± 26.5

107.3 ± 10.0

97.1 ± 30.7

48

100 ± 14.9

108.0 ± 7.9

88.5 ± 17.8

92.7 ± 12.5

HepG2

24

122.4 ± 16.5

135.1 ± 23.8

118.9 ± 13.2

146.3 ± 39.7

48

129.8 ± 8.5

129.6 ± 11.4

121.5 ± 7.9

125.1 ± 10.4

  1. The cells were treated with mevalonate for 24 and 48 h. The cell lysates were prepared and overall neutral SMase activities in the supernatant were determined. Results are expressed as means ± SD obtained from triple samples for each concentration in separate three experiments