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Table 3 Effects of chloroquine(CQ) intervention on resveratrol treatment in MCD medium induced-NASH

From: Resveratrol ameliorates hepatic steatosis and inflammation in methionine/choline-deficient diet-induced steatohepatitis through regulating autophagy

 

Control

MCD

MCD + R

MCD + R + CQ

MCD + CQ

ALT(U/L)

29.5 ± 4.6

83.6 ± 6.4*

38.5 ± 5.9

57.6 ± 7.3

94.7 ± 11.8▲▲

AST(U/L)

32.9 ± 3.1

100.2 ± 10.5*

51.0 ± 6.4

73.0 ± 8.1

112.5 ± 12.4

TG (μg/mg Pro)

86.7 ± 9.2

294.5 ± 28.9**

139.8 ± 16.2

229.6 ± 21.5

381.0 ± 36.1▲▲

TBARs (μmol/g Pro)

12.1 ± 1.2

43.5 ± 4.7**

23.9 ± 2.4

27.1 ± 2.6

53.6 ± 5.5▲▲

ROS

1.0 ± 0.2

6.6 ± 1.3**

2.4 ± 0.3

3.6 ± 0.7

9.8 ± 1.14▲▲

IL-1β (pg/ml)

25.1 ± 3.3

89.7 ± 12.3**

40.3 ± 4.3

68.3 ± 8.1

147.3 ± 13.3▲▲

IL-6 (pg/ml)

78.6 ± 5.8

285.5 ± 32.3**

185.1 ± 16.4

207.8 ± 21.6

358.6 ± 33.1▲▲

TNF-α (pg/ml)

121.3 ± 12.9

792.4 ± 82.7**

285.9 ± 25.7

541.3 ± 58.4

1043.9 ± 112.7▲▲

IL-1β mRNA

1.0 ± 0.1

3.2 ± 0.4**

1.3 ± 0.1

1.9 ± 0.2

7.4 ± 0.9▲▲

IL-6 mRNA

1.0 ± 0.2

5.7 ± 0.6**

2.2 ± 0.4

3.5 ± 0.4

8.9 ± 1.1▲▲

TNF-α mRNA

1.0 ± 0.1

8.7 ± 0.9**

1.7 ± 0.3

3.6 ± 0.5

14.3 ± 1.8▲▲

  1. Values are means ± SD, n = 3; MCD: methionine/choline-deficient medium
  2. R resveratrol (100 μmol/L), CQ chloroquine
  3. * P < 0.05, ** P < 0.01 compared to Control
  4. P < 0.05, P < 0.01 compared to MCD + R
  5. P < 0.05, ▲▲ P < 0.01 compared to MCD + R + CQ