Fig. 1

mRNA and protein expression of DDX5 assayed in 3T3-L1 preadipocytes and C3H10T1/2 mesenchymal stem cells induced to undergo adipogenesis for 8 days in vitro. mRNA expression of Ddx5 in (a) 3T3-L1 and (b) C3H10T1/2 cells differentiated for 8 days in vitro. Black bars indicate the peak of mRNA expression. Protein expression by western blotting for DDX5, PPARγ and GAPDH in (c) 3T3-L1 and (d) C3H10T1/2 cells differentiated for 8 days in vitro. Western blot bands were quantified using ImageJ. Transient knockdown using siRNA were performed on (e) 3T3-L1 and (f) C3H10T1/2 cells. g Cell viability assay using WST-1 was performed using cells treated with control siRNA (siControl), siRNA against Ddx5 (siDDX5) and bleomycin and compared to untreated cells. Data are represented as % cell viability compared to untreated cells. *p < 0.05, **p < 0.01, ***p < 0.001. Experiments were done in triplicates and error bars indicate SEM (n = 3). mRNA expression data and western blot densitometry were normalized to CycloA and GAPDH expression respectively