Fig. 2

Sustained knockdown of Ddx5 in 3T3-L1 cells differentiated for 8 days in vitro. (a) Cells were treated with siRNA continuously up to day 6 of differentiation and (b) viewed under a microscope and also stained for lipid accumulation using Oil Red O Stain. (c) Triglyceride accumulation was quantified and mRNA expression of (d) Ddx5, (e) Cebpb, (f) Pparg and (g) Lmna were investigated. All experiments were done in triplicates and statistical significance are denoted as follows: *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate SEM (n = 3). mRNA expression data were normalized to CycloA expression respectively