Fig. 4

Late knockdown of Ddx5 in 3T3-L1 cells differentiated for 8 days in vitro. (a) Cells were treated with siRNA only at D4 and D6 of differentiation and (b) viewed microscopically and also stained for lipid accumulation using Oil Red O stain. (c) Triglyceride accumulation was quantified and mRNA expression of (d) Ddx5, (e) Pparg and (f) Lmna were described. All experiments were done in triplicates and statistical significance are denoted as follows: *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate SEM (n = 3). mRNA expression data were normalized to CycloA expression respectively