Table 2 The SMARTpool siRNA ON-TARGETplus mouse siRNA (Thermo Scientific) designed to specifically target mouse Ddx5, Pparg and Cebpb. The SMARTpool siRNA consists of four individual duplexes designed to obtain a high level of gene silencing. The non-targeting siRNA is a negative control siRNA with at least four mismatches to any human mouse or rat gene
From: DDX5/p68 RNA helicase expression is essential for initiating adipogenesis
siRNA | siRNA details | Target sequence |
|---|---|---|
Ddx5 (13207) | Ddx5 -J-065333-09 | CAGUAAAGUUUUCGGGUUA |
| Â | Ddx5 -J-065333-10 | AAAUAAGACCUGAUAGGCA |
| Â | Ddx5 -J-065333-11 | GCUGAAUAUUGUCGAGCUU |
| Â | Ddx5 -J-065333-12 | ACAUAAAGCAAGUGAGCGA |
Pparg (19016) | Pparg -J-040712-05 | CGAAGAACCAUCCGAUUGA |
| Â | Pparg -J-040712-06 | ACCCAAUGGUUGCUGAUUA |
| Â | Pparg -J-040712-07 | UCACAAUGCCAUCAGGUUU |
| Â | Pparg -J-040712-08 | CGACAUGAAUUCCUUAAUG |
Cebpb (12608) | Cebpb -J-043110-09 | GAGCGACGAGUACAAGAUG |
| Â | Cebpb -J-043110-10 | CCUUUAGACCCAUGGAAGU |
| Â | Cebpb -J-043110-11 | GCACCCUGCGGAACUUGUU |
| Â | Cebpb -J-043110-12 | GAAAAGAGGCGUAUGUAUA |
Non-targeting siRNA #2 | D-001810-02-05 | UGGUUUACAUGUUGUGUGA |