Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

Poggi, Paola; Mirabella, Roberto; Neri, Simona; Assirelli, Elisa; Dolzani, Paolo; Mariani, Erminia; Calder, Philip C.; Chatgilialoglu, Alexandros

doi:10.1186/s12944-015-0166-3

Lipids in Health and Disease

Table 3 Membrane fatty acid profiles of Jurkat, Raji and THP-1 cell lines

From: Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

	Jurkat	Raji	THP-1
9:0	0,000 ± 0,000	0,046 ± 0,046	0,028 ± 0,062
12:0	0,056 ± 0,011	0,052 ± 0,015	0,062 ± 0,013
14:0	2,062 ± 0,085^ab	1,647 ± 0,093^ac	2,876 ± 0,064^bc
14:1n-5	0,047 ± 0,028	0,000 ± 0,000^c	0,062 ± 0,006^c
15:0	0,194 ± 0,012^ab	0,526 ± 0,074^ac	0,332 ± 0,013^bc
16:0	25,776 ± 2,014^b	23,959 ± 1,124^c	28,740 ± 0,998^bc
t16:1n-7	0,834 ± 0,036^ab	1,345 ± 0,108^ac	2,138 ± 0,022^bc
16:1n-7	7,188 ± 0,407^ab	1,656 ± 0,085^ac	5,331 ± 0,221^bc
17:0	0,411 ± 0,050^a	0,679 ± 0,071^ac	0,452 ± 0,013^c
17:1n-7	0,000 ± 0,000^ab	0,695 ± 0,05^ac	0,410 ± 0,014^bc
18:0	18,049 ± 0,606^ab	20,329 ± 0,837^ac	16,379 ± 1,056^bc
t18:1n-9	0,735 ± 0,033^ab	0,000 ± 0,000^a	0,000 ± 0,000^b
18:1n-9	24,938 ± 1,156^ab	20,063 ± 0,945^a	22,013 ± 0,585^b
18:1n-7	5,521 ± 0,356^ab	8,007 ± 0,437^ac	6,932 ± 0,205^bc
18:2n-6	3,144 ± 0,210^ab	3,893 ± 0,115^ac	1,499 ± 0,053^bc
18:3n-6	0,094 ± 0,017^ab	0,045 ± 0,013^a	0,042 ± 0,007^b
18:3n-3	0,302 ± 0,036^b	0,324 ± 0,05^c	0,124 ± 0,017^bc
20:0	0,608 ± 0,045^ab	0,421 ± 0,037^ac	0,476 ± 0,014^bc
20:1n-9	0,942 ± 0,091^ab	2,334 ± 0,091^ac	0,469 ± 0,010^bc
20:3n-9	0,302 ± 0,012^ab	0,635 ± 0,066^ac	1,005 ± 0,039^bc
20:3n-6	0,108 ± 0,007^ab	1,222 ± 0,042^ac	0,983 ± 0,069^bc
20:4n-6	2,166 ± 0,109^ab	4,347 ± 0,511^ac	3,501 ± 0,122^bc
20:3n-3	0,000 ± 0,000^ab	0,097 ± 0,013^a	0,094 ± 0,006^b
20:5n-3	0,042 ± 0,013^ab	0,572 ± 0,038^ac	0,509 ± 0,018^bc
22:0	0,946 ± 0,114^ab	0,409 ± 0,018^ac	0,455 ± 0,034^bc
22:1n-9	0,144 ± 0,018^ab	0,223 ± 0,024^ac	0,052 ± 0,006^bc
22:2n-6	0,000 ± 0,000^ab	0,117 ± 0,018^ac	0,077 ± 0,005^bc
23:0	0,056 ± 0,013	0,089 ± 0,015^c	0,040 ± 0,007^c
22:4n-6	0,747 ± 0,103^b	0,684 ± 0,092^c	0,231 ± 0,031^bc
22:5n-6	0,164 ± 0,026^b	0,216 ± 0,034	0,252 ± 0,020^b
22:5n-3	1,074 ± 0,121^ab	1,723 ± 0,069^ac	1,364 ± 0,038^bc
24:0	0,000 ± 0,000^a	0,073 ± 0,026^ac	0,000 ± 0,000^c
22:6n-3	1,479 ± 0,077^ab	2,835 ± 0,093^a	2,915 ± 0,117^b
24:1n-9	1,872 ± 0,190^ab	0,737 ± 0,071^ac	0,160 ± 0,024^bc
Ʃ SFA	48,158 ± 1,776	48,229 ± 1,23	49,839 ± 1,498
Ʃ MUFA	40,653 ± 1,636^ab	33,715 ± 1,303^a	35,429 ± 1,032^b
Ʃ PUFA	9,621 ± 0,343^ab	16,711 ± 0,464^ac	12,594 ± 0,465^bc
Ʃ trans FA	1,569 ± 0,034^ab	1,345 ± 0,108^ac	2,138 ± 0,022^bc
Ʃ Omega3	2,896 ± 0,153^ab	5,552 ± 0,145^ac	5,006 ± 0,157^bc
Ʃ Omega5	0,047 ± 0,028	0,000 ± 0,000^c	0,062 ± 0,006^c
Ʃ Omega6	6,422 ± 0,208^a	10,525 ± 0,555^ac	6,584 ± 0,271^c
Ʃ Omega7	12,709 ± 0,509^a	10,358 ± 0,511^ac	12,673 ± 0,435^c
Ʃ Omega9	28,199 ± 1,160^ab	23,992 ± 0,959^a	23,698 ± 0,648^b
UI	73,097 ± 2,362^ab	92,332 ± 1,687^ac	83,240 ± 2,764^bc
PI	43,162 ± 1,357^ab	69,931 ± 1,054^ac	62,191 ± 2,232^bc

Data are expressed as mean (± SD) weight % of total membrane fatty acids (n = 5). ^{a, b, c} Statistically significant difference (P < 0.05) as follows: ^a Jurkat vs Raji, ^b Jurkat vs THP-1, ^c Raji vs THP-1

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