Table 4 Comparison of membrane fatty acid profiles between immortalised cell lines and corresponding primary leukocyte classes

9:0

0,000 ± 0,000

0,000 ± 0,000

0,000 ± 0,000

0,046 ± 0,046

0,000 ± 0,000

0,000 ± 0,000

0,000 ± 0,000

12:0

0,056 ± 0,011

0,000 ± 0,000*

0,000 ± 0,000*

0,052 ± 0,015

0,000 ± 0,000*

0,041 ± 0,025

0,000 ± 0,000*

14:0

2,062 ± 0,085

0,635 ± 0,190*

0,870 ± 0,185*

1,647 ± 0,093

0,923 ± 0,215*

2,313 ± 0,493

0,413 ± 0,157*

14:1n-5

0,047 ± 0,028

0,000 ± 0,000*

0,000 ± 0,000*

0,000 ± 0,000

0,000 ± 0,000

0,041 ± 0,009

0,000 ± 0,000*

15:0

0,194 ± 0,012

0,256 ± 0,150*

0,412 ± 0,125*

0,526 ± 0,074

0,430 ± 0,139

0,324 ± 0,017

0,180 ± 0,107*

16:0

25,776 ± 2,014

27,156 ± 1,864

29,451 ± 2,352*

23,959 ± 1,124

31,780 ± 3,523*

28,591 ± 0,655

20,373 ± 2,321*

t16:1n-7

0,834 ± 0,036

0,265 ± 0,133*

0,364 ± 0,156*

1,345 ± 0,108

0,397 ± 0,171*

1,766 ± 0,074

0,203 ± 0,097*

16:1n-7

7,188 ± 0,407

0,257 ± 0,140*

0,341 ± 0,161*

1,656 ± 0,085

0,358 ± 0,174*

4,177 ± 0,195

0,255 ± 0,080*

17:0

0,411 ± 0,050

0,696 ± 0,169*

0,922 ± 0,258*

0,679 ± 0,071

0,879 ± 0,210

0,458 ± 0,076

0,435 ± 0,067

17:1n-7

0,000 ± 0,000

0,000 ± 0,000

0,000 ± 0,000

0,695 ± 0,050

0,000 ± 0,000*

0,436 ± 0,004

0,000 ± 0,000*

18:0

18,049 ± 0,606

26,877 ± 3,827*

28,296 ± 3,344*

20,329 ± 0,837

30,470 ± 4,447*

14,511 ± 0,640

24,146 ± 2,354*

t18:1n-9

0,735 ± 0,033

0,012 ± 0,033*

0,021 ± 0,060*

0,000 ± 0,000

0,020 ± 0,057

0,000 ± 0,000

0,039 ± 0,063

18:1n-9

24,938 ± 1,156

7,919 ± 1,053*

6,383 ± 1,044*

20,063 ± 0,945

7,083 ± 3,024*

22,967 ± 0,300

14,440 ± 1,039*

18:1n-7

5,521 ± 0,356

2,208 ± 0,375*

1,662 ± 0,424*

8,007 ± 0,437

1,431 ± 0,199*

7,906 ± 0,072

1,552 ± 0,225*

18:2n-6

3,144 ± 0,210

6,946 ± 1,323*

5,071 ± 0,803*

3,893 ± 0,115

4,705 ± 0,878

1,626 ± 0,161

6,577 ± 0,772*

18:3n-6

0,094 ± 0,017

0,179 ± 0,118

0,229 ± 0,138

0,045 ± 0,013

0,206 ± 0,183

0,037 ± 0,007

0,167 ± 0,091*

18:3n-3

0,302 ± 0,036

0,396 ± 0,319

0,601 ± 0,232*

0,324 ± 0,050

0,536 ± 0,280

0,130 ± 0,009

0,180 ± 0,090

20:0

0,608 ± 0,045

0,697 ± 0,241

1,096 ± 0,241*

0,421 ± 0,037

1,156 ± 0,215*

0,406 ± 0,026

0,533 ± 0,109

20:1n-9

0,942 ± 0,091

0,806 ± 0,475

1,141 ± 0,995

2,334 ± 0,091

0,896 ± 0,846*

0,572 ± 0,022

0,672 ± 0,249*

20:3n-9

0,302 ± 0,012

0,765 ± 0,090*

0,513 ± 0,116*

0,635 ± 0,066

0,608 ± 0,132

1,035 ± 0,033

0,618 ± 0,087*

20:3n-6

0,108 ± 0,007

1,519 ± 0,396*

1,009 ± 0,332*

1,222 ± 0,042

0,899 ± 0,318*

1,222 ± 0,030

1,357 ± 0,304*

20:4n-6

2,166 ± 0,109

12,755 ± 2,656*

9,251 ± 3,097*

4,347 ± 0,511

8,240 ± 4,173*

4,220 ± 0,060

16,422 ± 2,357*

20:3n-3

0,000 ± 0,000

0,041 ± 0,076

0,059 ± 0,119

0,097 ± 0,013

0,000 ± 0,000*

0,150 ± 0,015

0,025 ± 0,059*

20:5n-3

0,042 ± 0,013

0,098 ± 0,112

0,166 ± 0,301

0,572 ± 0,038

0,113 ± 0,165*

0,689 ± 0,017

0,138 ± 0,100*

22:0

0,946 ± 0,114

0,978 ± 0,579

1,642 ± 0,591*

0,409 ± 0,018

1,356 ± 0,435*

0,369 ± 0,085

0,492 ± 0,152

22:1n-9

0,144 ± 0,018

1,588 ± 0,595*

2,725 ± 1,570*

0,223 ± 0,024

2,014 ± 1,418*

0,067 ± 0,008

0,664 ± 0,430*

22:2n-6

0,000 ± 0,000

0,000 ± 0,000

0,024 ± 0,067

0,117 ± 0,018

0,000 ± 0,000*

0,074 ± 0,005

0,031 ± 0,045

23:0

0,056 ± 0,013

0,216 ± 0,277

0,239 ± 0,250

0,089 ± 0,015

0,087 ± 0,175

0,027 ± 0,013

0,057 ± 0,053

22:4n-6

0,747 ± 0,103

1,816 ± 0,314*

2,662 ± 1,945*

0,684 ± 0,092

1,545 ± 0,610*

0,278 ± 0,020

3,553 ± 0,446*

22:5n-6

0,164 ± 0,026

0,356 ± 0,110*

0,234 ± 0,189

0,216 ± 0,034

0,431 ± 0,754

0,296 ± 0,027

0,624 ± 0,227*

22:5n-3

1,074 ± 0,121

1,300 ± 0,444

0,855 ± 0,315

1,723 ± 0,069

0,726 ± 0,279*

1,653 ± 0,026

2,376 ± 0,445*

24:0

0,000 ± 0,000

0,557 ± 0,345*

0,809 ± 0,737*

0,073 ± 0,026

0,606 ± 0,292*

0,000 ± 0,000

0,307 ± 0,135*

22:6n-3

1,479 ± 0,077

2,672 ± 1,315

2,852 ± 2,253

2,835 ± 0,093

2,040 ± 1,799

3,481 ± 0,077

3,050 ± 1,187

24:1n-9

1,872 ± 0,190

0,035 ± 0,098*

0,098 ± 0,276*

0,737 ± 0,071

0,064 ± 0,180*

0,139 ± 0,017

0,123 ± 0,149

Ʃ SFA

48,158 ± 1,776

58,067 ± 5,419*

63,738 ± 5,544*

48,229 ± 1,230

67,687 ± 7,719*

47,040 ± 0,881

46,936 ± 4,789*

Ʃ MUFA

40,653 ± 1,636

12,812 ± 1,675*

12,351 ± 2,609*

33,715 ± 1,303

11,846 ± 3,172*

36,304 ± 0,505

17,705 ± 1,311*

Ʃ PUFA

9,621 ± 0,343

28,844 ± 4,890*

23,526 ± 4,114*

16,711 ± 0,464

20,049 ± 5,101*

14,890 ± 0,388

35,118 ± 3,867*

Ʃ trans FA

1,569 ± 0,034

0,276 ± 0,125*

0,385 ± 0,135*

1,345 ± 0,108

0,417 ± 0,172*

1,766 ± 0,074

0,241 ± 0,100*

Ʃ Omega3

2,896 ± 0,153

4,507 ± 1,587

4,533 ± 2,154

5,552 ± 0,145

3,415 ± 1,870

6,104 ± 0,126

5,770 ± 1,552

Ʃ Omega5

0,047 ± 0,028

0,000 ± 0,000*

0,000 ± 0,000*

0,000 ± 0,000

0,000 ± 0,000

0,041 ± 0,009

0,000 ± 0,000*

Ʃ Omega6

6,422 ± 0,208

23,572 ± 4,204*

18,481 ± 4,052*

10,525 ± 0,555

16,026 ± 5,418*

7,751 ± 0,274

28,730 ± 3,304*

Ʃ Omega7

12,709 ± 0,509

2,465 ± 0,376*

2,004 ± 0,477*

10,358 ± 0,511

1,789 ± 0,313*

12,519 ± 0,249

1,806 ± 0,291*

Ʃ Omega9

28,199 ± 1,160

11,112 ± 1,515*

10,861 ± 2,450*

23,992 ± 0,959

10,665 ± 3,083*

24,779 ± 0,333

16,516 ± 1,116*

UI

73,097 ± 2,362

109,691 ± 17,760*

92,754 ± 17,734*

92,332 ± 1,687

78,864 ± 20,728*

92,934 ± 1,835

141,417 ± 15,994*

PI

43,162 ± 1,357

91,565 ± 17,034*

79,091 ± 19,319*

69,931 ± 1,054

65,462 ± 16,533

73,093 ± 1,495

118,882 ± 15,487*