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Table 5 Membrane fatty acid profile comparison between freshly isolated and primary lymphocytes

From: Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

 

Freshly isolated lymphocytes

Primary lymphocytes 96 h - non stimulated

Primary lymphocytes 96 h - PHA stimulated

14:0

0,426 ± 0,024ab

0,678 ± 0,079ac

0,801 ± 0,062bc

15:0

0,196 ± 0,012ab

0,273 ± 0,058a

0,300 ± 0,055b

16:0

22,772 ± 1,171ab

28,499 ± 1,267ac

31,641 ± 1,304bc

t16:1n-7

0,261 ± 0,011

0,268 ± 0,028

0,316 ± 0,052

16:1n-7

0,281 ± 0,011ab

0,180 ± 0,011ac

0,233 ± 0,014bc

17:0

0,448 ± 0,025ab

0,717 ± 0,173a

0,770 ± 0,145b

17:1

0,065 ± 0,017ab

0,000 ± 0,000a

0,000 ± 0,000b

18:0

24,839 ± 1,710ab

30,630 ± 1,045a

32,467 ± 1,992b

t18:1n-9

0,000 ± 0,000

0,025 ± 0,049

0,041 ± 0,058

18:1n-9

15,653 ± 0,557ab

9,182 ± 0,084a

9,310 ± 0,882b

18:1n-7

2,076 ± 0,119ab

1,222 ± 0,027a

1,307 ± 0,209b

18:2n-6

6,512 ± 0,250ab

3,330 ± 0,085a

3,310 ± 0,231b

18:3n-6

0,077 ± 0,013ab

0,096 ± 0,006ac

0,130 ± 0,025bc

18:3n-3

0,117 ± 0,025a

0,254 ± 0,127a

0,255 ± 0,122

20:0

0,604 ± 0,040ab

1,106 ± 0,167a

1,153 ± 0,141b

20:1n-9

0,908 ± 0,073ab

0,517 ± 0,013a

0,524 ± 0,067b

20:3n-9

0,634 ± 0,036ab

0,333 ± 0,039a

0,402 ± 0,123b

20:3n-6

1,522 ± 0,083ab

1,137 ± 0,089a

1,024 ± 0,177b

20:4n-6

15,726 ± 0,900ab

12,940 ± 1,163ac

8,644 ± 1,592bc

20:5n-3

0,182 ± 0,018

0,227 ± 0,017c

0,170 ± 0,032c

22:0

0,301 ± 0,064ab

1,018 ± 0,371a

1,070 ± 0,441b

22:1n-9

0,082 ± 0,011ab

0,108 ± 0,015a

0,113 ± 0,023b

22:2n-6

0,080 ± 0,009b

0,117 ± 0,019c

0,000 ± 0,000bc

23:0

0,000 ± 0,000ab

0,167 ± 0,031a

0,185 ± 0,057b

22:4n-6

2,370 ± 0,104b

2,532 ± 0,294c

1,676 ± 0,303bc

22:5n-6

0,469 ± 0,075

0,686 ± 0,367

1,488 ± 2,231

22:5n-3

1,526 ± 0,076ab

1,558 ± 0,057ac

1,063 ± 0,174bc

22:6n-3

1,872 ± 0,094

2,197 ± 0,102c

1,606 ± 0,222c

Æ© SFA

49,588 ± 1,998ab

63,089 ± 1,234ac

68,386 ± 2,794bc

Æ© MUFA

19,065 ± 0,742ab

11,209 ± 0,068a

11,488 ± 1,106b

Æ© PUFA

31,086 ± 1,268ab

25,409 ± 1,258ac

19,769 ± 2,124bc

Æ© trans FA

0,261 ± 0,011

0,292 ± 0,072

0,357 ± 0,097

Æ© Omega3

3,697 ± 0,187b

4,236 ± 0,133c

3,094 ± 0,342bc

Æ© Omega5

0,000 ± 0,000

0,000 ± 0,000

0,000 ± 0,000

Æ© Omega6

26,756 ± 1,112ab

20,839 ± 1,209ac

16,272 ± 1,925bc

Æ© Omega7

2,422 ± 0,129ab

1,402 ± 0,018a

1,540 ± 0,220b

Æ© Omega9

17,276 ± 0,657ab

10,141 ± 0,069a

10,350 ± 0,928b

UI

124,585 ± 5,279ab

101,606 ± 4,870ac

80,302 ± 9,028bc

PI

98,379 ± 3,877ab

88,712 ± 4,192a

69,943 ± 11,107b

  1. Membrane fatty acid profile of freshly isolated lymphocytes compared to the membrane fatty acid profile of primary lymphocytes isolated, non-stimulated/PHA-stimulated and cultivated in vitro for 96 h. Data are expressed in weight % of total membrane fatty acids and presented as means ± SD (n = 5 for all). a, b, c Statistically significant difference (P < 0,05) as follows: a Freshly vs Non stimulated, b Freshly vs PHA-Stimulated, c Non stimulated vs PHA-Stimulated