Skip to main content

Table 6 Effect of lipid supplementation on membrane fatty acid profile of Jurkat cells

From: Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

 

CD4+

Jurkat

Jurkat + Refeed WT

12:0

0,000 ± 0,000

0,056 ± 0,011*

0,000 ± 0,000

14:0

0,635 ± 0,190

2,062 ± 0,085*

0,829 ± 0,155

14:1n-5

0,000 ± 0,000

0,047 ± 0,028*

0,000 ± 0,000

15:0

0,256 ± 0,150

0,194 ± 0,012*

0,172 ± 0,094

16:0

27,156 ± 1,864

25,776 ± 2,014

21,420 ± 2,863*

t16:1n-7

0,265 ± 0,133

0,834 ± 0,036*

0,236 ± 0,084

16:1n-7

0,257 ± 0,140

7,188 ± 0,407*

0,422 ± 0,229

17:0

0,696 ± 0,169

0,411 ± 0,050*

0,650 ± 0,299

18:0

26,877 ± 3,827

18,049 ± 0,606*

29,737 ± 2,063

t18:1n-9

0,012 ± 0,033

0,735 ± 0,033*

0,089 ± 0,142

18:1n-9

7,919 ± 1,053

24,938 ± 1,156*

11,018 ± 1,526*

18:1n-7

2,208 ± 0,375

5,521 ± 0,356*

2,104 ± 0,851

18:2n-6

6,946 ± 1,323

3,144 ± 0,210*

7,446 ± 1,034

18:3n-6

0,179 ± 0,118

0,094 ± 0,017

0,235 ± 0,132

18:3n-3

0,396 ± 0,319

0,302 ± 0,036

0,296 ± 0,101

20:0

0,697 ± 0,241

0,608 ± 0,045

0,753 ± 0,377

20:1n-9

0,806 ± 0,475

0,942 ± 0,091

0,634 ± 0,231

20:3n-9

0,765 ± 0,090

0,302 ± 0,012*

0,757 ± 0,091

20:3n-6

1,519 ± 0,396

0,108 ± 0,007*

2,110 ± 0,592

20:4n-6

12,755 ± 2,656

2,166 ± 0,109*

12,296 ± 1,326

20:3n-3

0,041 ± 0,076

0,000 ± 0,000

0,004 ± 0,009

20:5n-3

0,098 ± 0,112

0,042 ± 0,013

0,133 ± 0,114

22:0

0,978 ± 0,579

0,946 ± 0,114

0,448 ± 0,168*

22:1n-9

1,588 ± 0,595

0,144 ± 0,018*

0,081 ± 0,021*

22:2n-6

0,000 ± 0,000

0,000 ± 0,000

0,000 ± 0,000

23:0

0,216 ± 0,277

0,056 ± 0,013

0,258 ± 0,197

22:4n-6

1,816 ± 0,314

0,747 ± 0,103*

4,457 ± 0,385*

22:5n-6

0,356 ± 0,110

0,164 ± 0,026*

0,262 ± 0,068

22:5n-3

1,300 ± 0,444

1,074 ± 0,121

0,670 ± 0,072*

24:0

0,557 ± 0,345

0,000 ± 0,000*

0,069 ± 0,012*

22:6n-3

2,672 ± 1,315

1,479 ± 0,077

2,413 ± 1,232

24:1n-9

0,035 ± 0,098

1,872 ± 0,190*

0,000 ± 0,000

Æ© SFA

58,067 ± 5,419

48,158 ± 1,776*

54,337 ± 2,323

Æ© MUFA

12,812 ± 1,675

40,653 ± 1,636*

14,259 ± 1,005

Æ© PUFA

28,844 ± 4,890

9,621 ± 0,343*

31,078 ± 2,759

Æ© trans FA

0,276 ± 0,125

1,569 ± 0,034*

0,326 ± 0,180

Æ© Omega3

4,507 ± 1,587

2,896 ± 0,153

3,516 ± 1,119

Æ© Omega5

0,000 ± 0,000

0,047 ± 0,028*

0,000 ± 0,000

Æ© Omega6

23,572 ± 4,204

6,422 ± 0,208*

26,806 ± 2,474

Æ© Omega7

2,465 ± 0,376

12,709 ± 0,509*

2,526 ± 0,911

Æ© Omega9

11,112 ± 1,515

28,199 ± 1,160*

12,489 ± 1,447

UI

109,691 ± 17,760

73,097 ± 2,362*

126,496 ± 10,755

PI

91,565 ± 17,034

43,162 ± 1,357*

93,767 ± 11,347

  1. Membrane fatty acid profile of fresh CD4+ human T lymphocytes compared to the membrane fatty acid profile of traditionally cultured Jurkat cells and the membrane fatty acid profile of Refeed® WT supplemented Jurkat cells. Data are expressed in weight % of total membrane fatty acids and presented as means ± SD (n = 8 for CD4+, n = 5 for traditionally cultured Jurkat cells, n = 5 for Refeed® WT supplemented Jurkat cells). * Statistically significant difference (P < 0.05)