Fig. 1

15-HETE induced PAF phenotypic changes. PAFs were incubated with 15-HETE (0.5 μM) for 24 h. Subsequently myofibroblast markers, α-SMA protein expression, was determined as a percentage of the untreated controls using Western blot (a) and (b). The characteristics of myofibroblasts was assayed by immunocytochemistry staining; the nuclei was stained with 4′,6-diamidino-2-phenylindole (DAPI). Magnification: 200X. Data consists of the means of three independent experiments ± SEM (n = 3). * p < 0.05 vs. controls