Fig. 3

15-HETE-induced FGF-2 upregulation requires p38 MAPK. Cells were treated with 15-HETE (0.5 μM) for 24 h. a Western blot for p38 phosphylation and total p38 levels, and p-Egr-1 expression (b). c Cells treated with p38 specific inhibitor, SB203580 (10 μM), or transfection with Egr-1 siRNA (d), FGF-2 was analyzed using Western blot. Data consists of the means of three independent experiments ± SEM (n = 3). * p < 0.05 vs. controls; +p < 0.05 vs. 15-HETE