Fig. 5
FGF-2 induces α-SMA expression and cell proliferation. Cells were treated with FGF-2 (15 ng/ml) for 4 h. α-SMA protein expressions were determined by Western Blot (a) and Immunocytochemistry Staining. The nuclei were stained with DAPI. Magnification: 200X (b). c Cell viability was measured with MTT, and cell cyclin protein cyclin E (d) and p27kip1 (e) were assayed by Western blot. Cells were transferred with p27kip1 siRNAs, cell viability was measured with MTT (f), and cylin E was analyzed with Western blot (g). Data consists of the means of three independent experiments ± SEM (n = 3). *p < 0.05 vs. controls; +p < 0.05 vs. 15-HETE