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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Homology modeling and docking studies of ENPP4: a BCG activated tumoricidal macrophage protein

Fig. 5

a Analysis of ENPP4 cDNA sequence amplified by RT-PCR. b Enzyme restriction assay with restriction enzymes EcoRI and XhoI of pET-28a-ENPP4 vector construction. Lanes 1 shows the digested plasmids with its expected sizes released from constructed DNA-vectors. Lanes 2 shows the non-digested plasmids. c SDS gel electrophoretic patterns of recombinant ENPP4 after purification. Lanes 1–3: different concentration of ENPP4 protein stained by Coomassie blue. d PVDF membrane of western blotting assay of ENPP4 purified protein (32KD)

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Lipids in Health and Disease

ISSN: 1476-511X