(−)-Hydroxycitric acid reduced fat deposition via regulating lipid metabolism-related gene expression in broiler chickens

Table 6 Composition and nutrient levels of the experimental diets

Item	Starter	Finisher
Ingredient (%)
Corn	52.6	57..4
Wheat bran	2.0	4.0
Soybean meal	31.1	27.0
Fish meal	6.0	3.0
Calcium phosphate	1.0	1.5
Limestone	1.2	1.2
DL-Methionine	0.3	0.1
Vitamin-mineral premix^a	0.5	0.5
Rapeseed oil	5.0	5.0
NaCl	0.3	0.3
Nutrient composition Calculated
ME^b (kcal/kg)	3.10	3.14
CP (%)	22.52	19.74
Lys (%)	1.19	1.08
Met + cystine (%)	0.93	0.71
Ca (%)	1.00	0.9
Total P (%)	0.80	0.76
Available P (%)	0.47	0.39

Nutrient level of the diets was based on National Research Council recommendations
^aVitamin–mineral premix supplied the following per kilogram of diet: vitamin A, 1500 IU; vitamin D₃, 200 IU; vitamin E, 10 mg; vitamin K3, 0.5 mg; thiamine, 1.8 mg; riboflavin, 3.6 mg; D-pantothenic acid, 10 mg; folic acid, 0.55 mg; pyridoxine, 3.5 mg; niacin, 35 mg; cobalamin, 0.01 mg; biotin, 0.15 mg; Fe, 80 mg; Cu, 8 mg; Mn, 60 mg; Zn, 40 mg; I, 0.35 mg; and Se, 0.15 mg
^bME = metabolizable energy

ISSN: 1476-511X