Table 7 Prime sequence of targeted genes and β-actin
Gene | GenBank acession number | Primer sequences (5′–3′) | Orientation | Product size (bp) |
|---|---|---|---|---|
β-actin | L08165 | TGCGTGACATCAAGGAGAAG TGCCAGGGTACATTGTGGTA | Forward Reverse | 300 |
ACC | J03541 | GTTGTGGTTGGCAGAGCAAG GCACCAAACTTGAGCACCTG | Forward Reverse | 284 |
CPT-I | AY675193 | GGGTTGCCCTTATCGTCACA TACAACATGGGCTTCCGTCC | Forward Reverse | 151 |
FAS | NM205155 | TGAAGGACCTTATCGCATTGC GCATGGGAAGCATTTTGTTGT | Forward Reverse | 96 |
SREBP-1c | AY029224 | GTCGGCGATCCTGAGGAA CTCTTCTGCACGGCCATCTT | Forward Reverse | 105 |
ACLY | AJ851548 | CACCCAGAGGTGGATGTTCT GTTGCAGGCCCAATGTTAGT | Forward Reverse | 188 |
PPARα | AF470455 | CAAACCAACCATCCTGACGAT GGAGGTCAGCCATTTTTTGGA | Forward reverse | 64 |
AMPKα1 | DQ302133 | CAAGTAGTGTCTCGCACGGT GACTGATAGCTGGTCCCACG | Forward Reverse | 133 |
AMPKβ1 | DQ398947 | CACCAAGGATGGGGACAGAC TTCCAGATCCTGCTGCCAAG | Forward Reverse | 127 |
AMPKβ2 | DQ640761 | ACACTGCCTTCTTCTCCCTC GTGGGAGCTGAAAACACTGG | Forward Reverse | 195 |
AMPKγ1 | DQ133598 | AGGACTCCTTCAAGCCGTTG AACTCAGGCTTTGGGACCTC | Forward Reverse | 196 |