Fig. 5

a, b Luciferase assays of miR-106b binding sites on human STAT3 3′UTR. a Schematic representation of the 3′ untranslated region (3′UTR) of STAT3 mRNA with the predicted target sites for miR-106b. The seed sequences of miR-106b are in green letters, the target sites of STAT3 mRNA are in red letters, and the mutated miR-106b-binding sites are in gray boxes. b HUVECs were transfected with either wild-type 3′UTR STAT3 or mutant 3′UTR STAT3, along with the miR-106b mimic. hRluc (renilla luciferace gene) was reporter gene, while hluc (firefly luciferace gene) was reference gene as internal control. Results are shown as mean ± SEM representative of three independent experiments, there are significant differences in fluorescence intensity between STAT3-WT + NC transfection group and STAT3-WT + miR-106b mimic transfection group and also between STAT3-WT + miR-106b mimic transfection group and STAT3-MUT + miR-106b mimic transfection group.** P < 0.05, n = 3