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Table 3 Primers used for real-time PCRa

From: Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis

Gene

Genbank number

Primers position

Primers sequnce (5' to 3')

Annealing temperature(°C)

References

ACC

NM_205505

Forward

AATGGCAGCTTTGGAGGTGT

60.9

[23]

Reverse

TCTGTTTGGGTGGGAGGTG

FAS

J03860

Forward

CTATCGACACAGCCTGCTCCT

62.0

[23]

Reverse

CAGAATGTTGACCCCTCCTACC

CPT-1

AY675193

Forward

CAATGAGGTACTCCCTGAAA

57.5

[26]

Reverse

CATTATTGGTCCACGCCCTC

PPAR-α

AF163809

Forward

TGGACGAATGCCAAGGTC

60.3

[26]

Reverse

GATTTCCTGCAGTAAAGGGTG

SREBP-1c

AY029224

Forward

GAGGAAGGCCATCGAGTACA

60.3

[26]

Reverse

GGAAGACAAAGGCACAGAGG

ACOX1

NM_001006205

Forward

ATGTCACGTTCACCCCATCC

54.0

[21]

Reverse

AGGTAGGAGACCATGCCAGT

GADPH

NM_204305

Forward

GGTGAAAGTCGGAGTCAACGG

58.4

[57]

Reverse

CGATGAAGGGATCATTGATGGC

  1. a ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; CPT-1, carnitine palmitoyl transferase 1; PPAR-α, peroxisome proliferator activated receptor-alpha; SREBP-1c, sterol regulatory element binding protein 1; ACOX1, acyl CoA oxidase 1; GADPH, glyceraldehyde 3-phosphate dehydrogenase