Fig. 3

ApoA1 expression was suppressed by DNA methyltransferase inhibitor 5-aza-dC. a and b 5 CpG islands including two methylation CpG status in ApoA1 promotor were listed (a), the detection results of the 5 CpG islands methylation status were shown (b). HepG2.2.15 cells treated with 5 μM 5-aza-dC 48 h, ApoA1 mRNA and protein levels were detected by RT-PCR and Western blot respectively (c and d). Secretion of HBsAg and HBV particles in the supernatant were detected by ELISA and RT- PCR respectivly (e and f). Data are presented as the mean ± SD from three independent experiments. *p < 0.05 and **P < 0.01 compared with mock