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Table 5 Primer sequences used for the real-time PCR analysis

From: Maternal diet of polyunsaturated fatty acid altered the cell proliferation in the dentate gyrus of hippocampus and influenced glutamatergic and serotoninergic systems of neonatal female rats

Gene (accession no.)

Sense Primer (5’-3’)

Antisense Primer (5’-3’)

Amplicon length

GAD65 (NM_012563.1)

CAGCCTGTGAAGGAGAAAGG

GGTCTGCCAATTCCCAATTA

159 bp

GAD67 (M76177.1)

CTGGAGCTGGCTGAATACCT

TCGGAGGCTTTGTGGTATGT

146 bp

TPH2 (NM_173839.2)

GGGTTACTTTCCTCCATCGGA

AAGCAGGTTGTCTTCGGGTC

119 bp

GS (M29579.1)

CCACTGTCCCTGGGCTTAGTTTA

AGTGACATGCTAGTCCCACCAA

85 bp

TH (NM_012740.3)

CTCCTCACCTATGCATTCAC

TCCAATGTCCTGGGAGAACT

105 bp

β-Actin (NM031144)

CATCCTGCGTCTGGACCTGG

TAATGTCACGCACGATTTCC

116 bp

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Lipids in Health and Disease

ISSN: 1476-511X