Fig. 5

a Western blot detected the expression of Wnt signaling pathway key molecules. Hypoxia and reoxygenation (H+R) caused a marked increase in the protein levels of Dvl, β-catenin, and c-Myc. These changes were antagonized by Sfrp1-transfected cells before hypoxia and reoxygenation (Sfrp1+H+R); however, pre-treatment with the Wnt signaling pathway activator, Licl, increased the expression of Dvl, β-catenin, and c-Myc. b The median value of Dvl/GADPH with interquartile range (box) and range (whiskers). c The median value of β-catenin/GADPH with interquartile range (box) and range (whiskers). d The median value of c-Myc/GADPH with interquartile range (box) and range (whiskers). Post-hoc analyses of group differences were assessed with Kruskal-Wallis pairwise comparison. Statistical significance was set at the standard level (p<0.05), and the Bonferroni correction was applied in post-hoc analyses (α=0.05/6=0.0083). (Note:* vs. control P<0.001; # vs. H+R P<0.001; vs. Sfrp1+H+R P<0.001)