Fig. 1

Diabetic HDL and diabetic nephropathy HDL are less efficient in promoting HUVEC migration. a HUVEC monolayers were scratched by manual scraping and treated with PBS(C), N-HDL (N), D-HDL (D), or DN-HDL (DN) respectively at 100 ug/ml apoA-I for 10 h. Migration into the wound was photographed (50X objective lens). The red line indicated the scratch edge. b Distance between gaps was measured, and the results were expressed as percentage of various HDL-treated cells in comparison with control. c HUVECs were treated with PBS(C), N-HDL (N), D-HDL (D), or DN-HDL (DN) at 100 ug/ml apoA-I for 10 h respectively in transwell assay, and pictures were taken in six random high-power (50X) fields. d Migratory cells were counted, and the results were expressed as percentage of various HDL-treated cells in comparison with control. Each experiment was conducted in duplicate in three repeats and HDL were pooled from six individuals with different patients in each repeat. (n = 12 in control group, n = 18 in diabetes group, and n = 18 in diabetic nephropathy group, **p < 0.01; ***p < 0.001; one-way ANOVA)