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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution

Fig. 2

PAF-AH activity and protein expression in white and black women. PAF-AH activity of diluted sera was measured at A412 over a 20 min time interval using the PAF Acetylhydrolase Assay Kit. One unit of activity is defined as 1 μmol of substrate disintegrated per minute (a). Isolated HDL (bc) and participant sera (de) were run on reducing 12.5 % SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with rabbit anti-PAF-AH antibody. Results are representative of randomized experiments (b) and (d). Densitometry of PAF-AH expression in HDL (c) and sera (e) Results represent means ± SEM * p < 0.05

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