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Table 1 Ingredients and concentrations of individual tocopherols in the experimental diets

From: Conjugated linoleic acid influences the metabolism of tocopherol in lactating rats but has little effect on tissue tocopherol concentrations in pups

  Control diet CLA diet
Ingredient, g/kg diet
 Corn starch 532 532
 Casein 200 200
 Sucrose 100 100
 Cellulose 50 50
 Soybean oil 55 55
 Sunflower oil 15 -
 CLA supplement (Lutalin®) - 15
 Mineral mixa 35 35
 Vitamin mixa 10 10
 L-Cysteine 3 3
Dietary tocopherol concentration (μmol/kg diet)b
 α-tocopherol 140 ± 3.28 141 ± 2.02
 γ-tocopherol 60.4 ± 1.30 59.8 ± 1.43
 δ-tocopherol 6.57 ± 0.37 6.58 ± 0.22
 Tocopherol equivalentsc 147 ± 3.28 147 ± 2.01
  1. aThe mineral and vitamin mix were composed as recommended for the AIN 93G diet by Reeves et al. [11]
  2. bmeans ± standard deviations of 5 analyses of feed samples collected in weeks 1 to 5. The β- and δ-tocopherols, which are quantitatively negligible as source of active vitamin E in soybean and sunflower oil [32], could not be separated by HPLC and are summarized as δ-tocopherol
  3. csum of active α, δ- and γ-tocopherol considering conversion factors of 0.10 for γ-tocopherol and 0.03 for δ-tocopherol as compared to α-tocopherol (100 % active) [17]